小鼠脾切除对肠源性感染影响的实验研究

Study of enterogenic infection after splenectomy in mice

目的 研究小鼠脾脏在肠源性感染中的作用。方法 对脾切除或假脾切除的 139只小鼠进行了以下观察 :①小肠粘膜的病理变化 ;②组织的细菌培养和荧光标记菌的检测 ;③血浆内毒素、肠粘膜IgA与肠源性感染的关系。结果 脾切除动物近 6 0d内 ,小肠组织的细菌培养和荧光标记菌检测的阳性率极低 ,无统计学意义。 90d后 ,小肠组织的细菌培养和荧光标记菌检测的阳性率显著高于对照组 ,小肠粘膜出现了明显的病理变化 ,血浆内毒素水平升高 ,肠粘液IgA降低。结论 脾切除的后期引起了明显的肠道肠源性感染。因此 ,脾创伤时 。

Objective To study the role of spleen in enterogenic infection in mice. Methods One hundred and thirty nine Kunming strain mice were splenectomized or sham operated on. Then the following items were studied: ①pathologic changes of ileal mucosa. ②culture for bacteria and detection of the bacteria labeled with fluorescein in different organs. ③relationship of plasma level of endotoxin and IgA in intestinal mucus with enterogenic infection in different organs. Results Sixty days after splenectomy, the positive rate of the cultured bacteria and detection of fluorescein labeled bacteria showed no statistical difference between the experimental group and the control. Ninety days after splenectomy, the positive rate of cultured for bacteria and detection of fluorescein labeled bacteria was increased, the plasma level of endotoxin elevated, IgA in intestinal mucus decreased and detachment of intestinal epithelium and damage of intestinal villi were found.Conclusion The results suggest that significant intestinal bacteria translocation occurs 90 days after splenectomy. It is considered that a surgeon should try his best to preserve the spleen when he deals with patients with injured spleen.