多靶标生物标志物检测的微流体磁敏生物传感器研制

Development of Integrated Microfluidic Magnetic Biosensor for Multi-biomarker Detection

将磁敏传感器与微流体测试卡集成,研制了可快速检测多靶标生物标志物的微流体免疫磁敏传感器。以3种消化系统肿瘤标志物(甲胎蛋白、癌胚抗原、糖链抗原)为模型靶标,对免疫反应各步反应的液体流速、免疫反应时间以及反应后的冲洗速度进行了优化,评价了多靶标同时检测的系统性能。在微流体磁敏生物传感器系统中,建立了血清样本中3种靶标同时检测的标准工作曲线,AFP、CEA和CA19-9的检出限分别达到0.1μg/L、0.1μg/L和30 U/mL,线性范围跨越4个数量级。微流体磁敏生物传感器可在30 min内完成多靶标生物标志物检测,临床血清样本的测试结果与ELISA法一致,具有检测时间短、灵敏度高的优点。

By integrating magnetic sensors and microfluidic system,a microfluidic magnetic bioprocessor was developed for the rapid determination of multi-target biomarkers.Three digestive system tumor makers（α-fetoprotein（AFP）,carcinoembryonic antigen（CEA）,carbohydrate antigen 19-9,（CA19-9） were simultaneously detected in the microchannel as a model system to evaluate this multi-target detection system performance.The experiment parameters including injecting flow velocity,the immune reaction time and rinsing rate for each step were optimized.In the microfluidic magnetic biosensor,standard curves were established for the detection of three tumor markers in serum samples,with the detection limits of 0.1 μg/L（CEA）,0.1 μg/L（AFP）,30 U/mL（CA19-9） and the dynamic ranges spanning four orders of magnitude.Multi-biomarker analysis could be completed within 30 min,and good linear correlations were obtained between the results of magnetic biosensor and ELISA method,while the former was with the advantage of fast detection and high sensitivity.