生物芯片波导共振光散射扫描装置的研制

Development of Light Waveguide Resonance Light-Scattering Scanner for Microarray Detection

以光波导(Optical/Light waveguide)作为激发方式,利用共振光散射(Resonance light-scattering,RLS)原理,研制微阵列生物芯片(Microarray)波导共振光散射扫描装置。此装置主要由光源部件、波导激发部件、光电转换部件、机械传动部件和上位机软件组成。其最小扫描分辨率可达5μm,最大扫描范围100 mm。通过使用新的激发方式,有效提高了RLS的信噪比。使用白光作为激发光源,降低了设备的成本。对金纳米粒子标记的单糖芯片、DNA芯片和多肽芯片的检测结果表明,此装置对溶液中的伴刀豆球蛋白凝集素(ConA)和靶标DNA的检出限分别为1 ng/mL和100 pmol/L,对芯片表面固定的多肽的检出限为100 fg,较现有的商品生物芯片扫描仪降低1～2个数量级。本装置结构简单,成本低廉。

An optical waveguide resonance light-scattering scanner（RLS Scanner） was developed for microarray detection.The major components of the device include optical system,waveguide excitation system,photovoltaic conversion system,a mechanical drive system and the PC software.The minimum scanning resolution of the RLS scanner is 5 μm,and the maximum scanning range is 100 mm.The signal-to-noise ratio（S/N） of RLS can be improved by the waveguide excitation mode.This device is capable of using a white light source as the excitation source,thereby providing significant cost-savings.Carbohydrate microarray,DNA microarray and peptide microarray were used to demonstrate the detection performance.Detection limits of 1 μg/L and 100 pmol/L in solution were obtained for concanavalin A（ConA） and DNA respectively.For the peptide on microarray spots,a detection limit of 100 fg was reported.The RLS scanner showed at least one to two orders of magnitude more sensitive than that of commercial scanner.Furthermore,taking advantage of the RLS technique,the device was characterized by simple construction and low cost.