期刊文献+

GSK-3β、PTEN、PLK1在儿童急性髓系白血病中的表达及意义 被引量:3

Expression and clinical significance of GSK-3β,PTEN,PLK1 in pediatric acute myeloid leukemia
下载PDF
导出
摘要 目的探讨糖原合酶激酶-3β(GSK-3β)、与张力蛋白同源的位于第10号染色体磷酸酶基因(PTEN)、Polo样激酶1(PLK1)在儿童急性髓系白血病(AML)中的表达及意义。方法 RT-PCR方法检测实验组(33例初诊AML患儿的骨髓)和对照组(10例正常骨髓)骨髓单个核细胞(BMMNC)中GSK-3βmRNA、PTEN mRNA、PLK1mRNA的表达,ELISA方法检测GSK-3β蛋白及P-GSK-3β的表达。结果实验组中GSK-3βmRNA、GSK-3β蛋白、PLK1mRNA的表达量高于对照组(P=0.012;P=0.014;P=0.040);PTEN mRNA、P-GSK-3β的表达量低于对照组(P=0.012;P=0.002)。GSK3β蛋白与PTEN mRNA的表达呈负相关(r=-0.415,P=0.016);GSK-3βmRNA、GSK3β蛋白与PLK1mRNA的表达成呈正相关(r=0.388,P=0.026;r=0.427,P=0.013)。外周血白细胞计数增高者GSK-3β蛋白表达增高;临床危险度高者GSK-3βmRNA、GSK-3β蛋白的表达增高,P-GSK-3β的表达降低。结论在儿童AML中,GSK-3β和PLK1可能起癌基因的作用,PTEN可能起抑癌基因的作用。 Objective To explore the expression and clinical significance of GSK-3β, PTEN and PLK1 in pediatric AML. Methods Experiment group was bone marrows of 33 cases incipient children with AML. Control group was 10 cases normal bone marrows. GSK-3β,PTEN and PLK1 gene expressions in BMMNC of the two groups were tested using semi quantitative reverse transcriptase polymerase chain reaction(RT-PCR)teehnique. G SK-3β protein and P-GSK-3β expressions were tested by ELISA. Re- subs The expression of GSK-3β mRNA,GSK-3β protein,PLK1 mRNA in BMMNC of children with AML was higher than that of control group(P= 0. 012 ; P = 0. 014; P = 0. 040) ; The expression of PTEN mRNA, P-GSK-3β was lower than the control group (P= 0. 012; P= 0. 002) ; GSK-3β protein had a negative correlation with PTEN mRNA(r= - 0. 415, P = 0. 016) ; GSK-3βmRNA, GSK-3β protein had a positive correlation with PLK1 mRNA(r= 0. 388, P=0. 026;r= 0. 427, P= 0. 013). The expression of GSK-3β protein was high in which had high peripheral white blood cell counts;both the expressions of GSK-3β mRNA and GSK-3β pro- tein were high in which had high risklbut the expression of P-GSK-3βwere low. Conclusion In pediatric AML,GSK-3β and PLK1 may play a role of oncogene and PTEN may play a role of tumor suppressor gene.
出处 《重庆医学》 CAS CSCD 北大核心 2013年第28期3347-3349,共3页 Chongqing medicine
关键词 GSK-3Β PTEN PLK1 前体细胞淋巴母细胞白血病淋巴瘤 GSK-3β PTEN PLK1 precursor cell lymphoblastic leukemia-lymphoma
  • 相关文献

参考文献16

  • 1Pui CH, Carroll WL, Meshinchi S, et al. Biology, riskstratification,and therapy of pediatric acute leukemia: anupdate[J]. J Clin Oncol,2011 .29(5) :551-565.
  • 2Gum RJ,Gaede LL, Koterski SL,et al. Reduction of pro-tein tyrosine phosphatase IB increases insulin-dependentsignaling in ob/ob mice[J], Diabetes,2003 ,52(1) : 21-28.
  • 3Gandy JC, Melendez-Ferro M, Bijur GN,et al. Glycogensynthase kinase-3(3(GSK3p) expression in a mouse modelof Alzheimer's disease: a light and electron microscopystudy [J]. Synapse,2013,67(6) :313-327.
  • 4Miyashita K, Nakada M, Shakoori A, et al. An emergingstrategy for cancer treatment targeting aberrant glycogensynthase kinase 3 beta [J]. Anticancer Agents MedChem,2009,9(10) :1114-1122.
  • 5Al-Khouri AM, Ma Y, Togo SH, et al. Cooperative phos-phorylation of the tumor suppressor phosphatase and ten-sin homologue (PTEN) by casein kinases and glycogensynthase kinase 3beta [J]. J Biol Chem,2005,280 (42):35195-35202.
  • 6沈权,陈泽,刘旭萍,邢海燕,王敏,王建祥.抑癌基因PTEN mRNA在白血病细胞中的表达及意义[J].中华血液学杂志,2005,26(8):493-496. 被引量:20
  • 7Gauffin F, Diffner E, Gustafsson B, et al. Expression ofPTEN and SHP1,investigated from tissue microarrays inpediatric acute lymphoblastic, leukemia[J]. Pediatr He-matol Oncol,2009 ,26(1) :48-56.
  • 8Wasch R, Hasskarl J, Schnerch D,et al. BI_2536 — targe-ting the mitotic kinase Polo-like kinase 1 (Plkl) [J], Re-cent Results Cancer Res,2010,184:215-218.
  • 9周琼,白明,辛建保,陶晓南,金阳,苏远.氯化锂抑制A549细胞增殖及其对Polo样激酶1转录活性的影响[J].中国生物化学与分子生物学报,2006,22(10):832-838. 被引量:2
  • 10Holmes T, O' Brien TA,Knight R, et al. Glycogen syn-thase kinase-3beta inhibition preserves hematopoieticstem cell activity and inhibits leukemic cell growth [J].Stem Cells,2008,26(5) :1288-1297.

二级参考文献31

  • 1Tamura M, Gu J, Matsumoto K, et al. Inhibition of cell migration,spreading, and focal adhesions by tumor suppressor PTEN. Science,1998, 280: 1614-1617.
  • 2Maehama T, Dixon JE. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem, 1998, 273:13375-13378.
  • 3le Good JA, Ziegler WH, Parekh DB, et al. Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1. Science, 1998, 281: 2042-2045.
  • 4Wu RC, Li X, Schonthal AH. Transcriptional activation of p21WAF1by PTEN/MMAC1 tumor suppressor. Mol Cell Biochem, 2000, 203:59-71.
  • 5Zhu X, Kwon CH, Schlosshauer PW, et al. PTEN induces G ( 1 )cell cycle arrest and decreases cyclin D3 levels in endometrial carcinoma cells. Cancer Res, 2001,61:4569-4575.
  • 6Moon SK, Kim HM, Kim CH. PTEN induces G1 cell cycle arrest and inhibits MMP-9 expression via the regulation of NF-kappaB and AP-1 in vascular smooth muscle cells. Arch Biochem Biophys, 2004,421:267-276.
  • 7Terakawa N, Kanamori Y, Yoshida S. Loss of PTEN expression followed by Akt phosphorylation is a poor prognostic factor for patients with endometrial cancer. Endocr Relat Cancer, 2003,10: 203-208.
  • 8Mao C D,Hoang P,DiCorleto P E.Lithium inhibits cell cycle progression and induces stabilization of p53 in bovine aortic endothelial cells[J].J Biol Chem,2001,276(28):26180-26188
  • 9Smits V A,Essers M A,Loomans D S,et al.Inhibition of cell proliferation by lithium is associated with interference in cdc2activation[J].FEBS Lett,1999,457(1):23-27
  • 10Uchiumi T,Longo D L,Ferris D K.Cell cycle regulation of the human polo-like kinase (PLk) promoter[J].J Biol Chem,1997,272(14):9166-9174

共引文献20

同被引文献33

引证文献3

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部