青果多酚对人宫颈癌Hela细胞增殖与凋亡的影响

Effect of polyphenol from Fructus Canarii on proliferation and apoptosis of Hela cells

目的:研究青果多酚对体外培养的人宫颈癌Hela细胞增殖与凋亡的影响。方法:利用超声波提取青果多酚,分光光度法检测其含量,采用MTT法、免疫细胞化学法、流式细胞术检测Hela细胞增殖抑制率与凋亡情况。结果:青果多酚对Hela细胞的增殖有明显抑制作用,并存在时间和剂量依赖性;青果多酚可通过激活caspase-3促进细胞的凋亡。结论:青果多酚对Hela细胞有抑制增殖和促进凋亡的作用。

Objective： To study the effect of polyphenol from Fructus Canarii on proliferation and apoptosis of Hela cells in vitro. Methods： Polyphenol from Fructus Canarii was extracted by ultrasonic ethanol method and was tested by spectrophotometry with Folin-Ciocalteu reagent and standard of Gallic acid. The effect on proliferation and apoptosis in Hela cells was tested by MTT assay, immunocytochemical methods, and flow cytometry. Results： Polyphenol from Fructus Canarii could obviously inhibit the proliferation of Hela cells in a time-and dose-dependent manner. Polyphenol could induce apoptosis by stimulating caspase-3. Conclusion：Polyphenol from Fructus Canarii could inhibit proliferation and induce apoptosis of Hela cells in vitro.