巨细胞病毒肺炎肺泡灌洗液中IFN-γ、IL-6的水平观察

Observation of change of IFN-γ and IL-6 in bronchoalveolar lavage fluid of cytomegalovirus pneumonia

目的检测巨细胞病毒(MCMV)肺炎小鼠的肺泡灌洗液(BALF)中炎症细胞因子IFN-γ、IL-6的水平,观察其水平变化并探讨临床意义。方法建立MCMV肺炎模型,HE法观察肺组织病理,PCR及凝胶电泳法检测MCMV-DNA,ELASA法测定IFN-γ、IL-6的水平。结果 MCMV肺炎组病理提示为间质性肺炎,更昔洛韦治疗后炎症有减轻;MCMV肺炎组小鼠的肺组织及BALF中均存在MCMV-DNA;较B组,C组IFN-γ水平下降,IL-6升高,而更昔洛韦治疗后,IFN-γ上调,IL-6降低,这两细胞因子在肺组织及BALF中的变化趋势一致,且各组间比较,差异均有统计学意义(P<0.05)。结论检测肺泡灌洗液中IFN-γ、IL-6的水平变化对临床诊断、治疗及预后有一定的指导价值。

Objective To investigate the clinical significance of IFN-γ and IL-6 in bronchoalveolar lavage fluid of mice with cytomegalovirus pneumonia. Methods The model of MCMV pneumonia was established, and the pathological changes of lung tissues were observed by HE method. MCMV-DNA was detected by PCR and electrophoresis. The levels of IFN-γ and IL-6 were detected by ELISA. Results The pathological results revealed that MCMV infection was interstitial pneumonia. The lung injury was lighten after the treatment of ganciclovir. MCMV-DNA existed in lung tissues and BALF. The level of IFN-γ decreased obviously, and the level of IL-6 increased in the group C while compared with the group B. Conclusion The detection of IL-6 and IFN-γ, in the brenchoalveolar lavage fluid has certain clinical value for the diagnosis, therapy and prognosis.