直接涂片法与改良法对抗酸杆菌检出率的比较

Comparison of direct smear method and improvement method to detection rate of ant-bacillus

目的探讨一种简单易行,提高阳性率的胸水涂片抗酸杆菌检查方法。方法采用破坏红细胞改良法和直接涂片法对96例随机采样的胸水标本进行抗酸染色镜检,其中直接涂片法只采用离心沉淀涂片,改良法采用蒸馏水破坏红细胞后取离心沉淀物涂片。结果直接涂片法抗酸染色阳性率为3.13%,破坏红细胞改良法抗酸染色阳性率为10.42%(P<0.05)。结论破坏红细胞改良法抗酸染色阳性检出率相比较直接涂片法有显著性差异,且涂片效果清晰,易于辨认,故其可作为综合性基层医院一种行之有效的检测方法推广应用。

Objective To approach an ant-bacillus check method of pleural fluid smear which is simple and would raise the positive rate.Methods To deploy the improvement method of demolishing red cells and direct smear method to give an acid-fast stain microscopy to 96 cases of pleural fluid collection of random sampling Cuj.The direct smear method only deploys centrifugation precipitate smear method,and the improvement method extracts centrifugation precipitate after deploying distilled water to destroy red cells.Results The positive rate of acid-fast stain of direct smear method is 3.13%,and that of the improvement method of demolishing red cells is 10.42%（P＆lt;0.05）.Conclusion The positive rate of damage there was significantly difference between red blood cells modified acid-fast staining,and smear effect is clear,easy to identify,The destruction of red blood cells modified method can be used as a comprehensive grass-roots hospital a effective method,and application.