模拟高原低氧对大鼠心肌线粒体中钙浓度的影响及中药的保护作用

Effect of simulating plateau hypoxia on myocardium mitochondria Ca2 +density and protective action of compound Chinese herbs in rats

目的观察急进高原及持续低氧对大鼠心肌线粒体中钙离子浓度的影响,探讨中药复方对心肌线粒体的保护作用。方法 SD雄性大鼠分为平原对照组(C)、急进高原1 d组(H1)、中药干预1 d组(Z1)、急进高原3 d组(H3)、中药干预3 d组(Z3)、急进高原7 d组(H7)、中药干预7 d组(Z7)。用荧光分光光度法测线粒体内游离Ca2+浓度;采用分光光度法测线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶。结果与C组比较,急进高原的3组线粒体内Ca2+浓度明显降低(P<0.001),7 H组线粒体膜钠钾泵活性、钙镁泵活性明显降低(P<0.05)。相等时间段比较表明,Z3组、Z7组线粒体内Ca2+浓度明显升高(P<0.001)。Z7组钠钾ATP酶活力明显升高(P<0.05)。结论急进高原使大鼠心肌线粒体中钙浓度明显降低,中药复方改善了低氧对线粒体的影响。

[ Objective] To investigate the effect of rush entry into plateau and continuous hypoxia on Ca2＋ density in myocardium mitoehondria, and explore the protective action of compound Chinese herbs in rats. [ Methods ] SD male rats were divided into plain control group （ C ） , plateau hypoxia 1 day group （H1 ）, the traditional Chinese medicine intervention 1 day group （ Z1 ）, plateau hypoxia 3 day group （ H3 ）, the traditional Chinese medicine intervention 3 day group （ Z3 ）, plateau hypoxia 7 day group （ I-I7 ）, the traditional Chinese medicine intervention 7 day group （Z7）. Ca2 ＋ density in myocardium mitoehondria was measured with fluo- rospectrophotometry. Na ＋ -K ＋ ATPase and Ca2 ＋ -Mg2 ＋ -ATPase in myocardium mitoehondria were measured with spectrophotometry. [Results] Compared with C group, Ca2＋ density in myocardium mitochondria was decreased significantly in H3 groups P 〈 0. 001 ）, activity of Na ＋ -K ＋ -ATPase and Ca2＋ -Mg2＋ -ATPase in myocardium mitochondria was decreased significantly in I-I7 group （P 〈 0.05 ）. Ca2 ＋ density in myocardium mitochondria was significantly increased both in the Z3 group and the Z7 group I P 〈0. 001 ）. The activity of Na＋-K ＋ -ATPase of myocardium mitochondria was significantly increasesd in the Z7 group （P 〈 0.05 ）. [ Conclusion] Ca2＋ density in myocardium mitochondria is decreased during rush entry into plateau in rats, effect of hypoxia on myocardium mitochondria is relieved by the compound Chinese herbs.