摘要
目的探讨番茄红素对体外培养的人脑胶质瘤U251细胞增殖及诱导凋亡的作用。方法用CCK-8法检测不同浓度番茄红素作用于U251细胞24 h、48 h及72 h后细胞的存活率;应用流式细胞术(FCM)检测细胞周期。结果番茄红素能抑制人脑胶质瘤U251细胞的生长,呈量-效及时-效关系(P<0.01);FCM检测表明,细胞主要被阻滞在G0/G1期;番茄红素诱导细胞凋亡率较对照组明显增加(P<0.01),具有浓度依赖性。结论番茄红素能抑制U251细胞增殖,诱导细胞凋亡。
Objective To discuss the anti-proliferation and inducing apoptosis effects of lycopene on human glioma U251 cells in vitro. Methods The survival rate of U251 cells after 24 h,48 h and 72 h exposure tO different concen- trations of lycopene were detected by CCK-8 ;The cell cycle was detected by flow cytometry（FCM）. Results Lycopene could inhibit the growth of human glioma U251 cells,and had dose-effect and time-effect relationship（P〈0.01 ）;FCM testing showed that the ceils were mostly blocked in G0/G1 stage ;The rate of cell apoptosis induced by lycopene was significantly higher than that in the control group（P〈0.01 ）, and it was concentration-dependent. Conclusion Lycopene can inhibit the proliferation of U251 cells and induce cells apoptosis.
出处
《中国现代医生》
2013年第27期3-4,8,共3页
China Modern Doctor
