利用分子标记辅助选育白菜薹复等位基因型雄性不育系

The Directional Transfer of a Multiple-allele Male Sterile Line in Chinese Cabbage by using Molecular Marker-assisted Selection

为解决白菜薹杂种优势利用中的杂交制种手段问题,以大白菜复等位基因型雄性不育系为不育源,设计定向转育方案,采用连续回交的方法转育不育性和农艺性状,同时利用分子标记辅助选择目标基因型植株,向白菜薹自交系中转育不育基因,创制白菜薹雄性不育系。通过26对SSR引物的筛选鉴定,获得与显性雄性不育基因Ms紧密连锁、且同样可以标记同一位点恢复基因Msf和可育基因ms的分子标记GSSR1。经过3个世代的回交转育,创制出了具有100%不育度和100%不育株率的白菜薹复等位基因型雄性不育系BGMS-3。以BGMS-3为母本,与6个白菜薹自交系杂交,筛选出1个强优势组合C3。

In order to create an approach for utilization ofheterosis in Chinese cabbage （Brassica campestris ssp. chinenesis L.）, a breeding method for multiple-allele male sterile line and a ＂directional transfer program＂ was designed and carried out in the experiment. A multiple-allele male sterile line of Chinese cabbage ＂Wangzi＂ was used as a resource of male sterility, and an inbred line of Chinese cabbage was the target line. Recurrent backcrossing method was employed to transfer male sterility and horticultural characters while the genotype was identified through molecular marker-assisted selection. The GSSR1, which is closely linked to the male sterility gene ＂Ms ＂, was chosen from 26 pairs of SSR primers to apply in the selection. GSSR1 also could select the fertile gene ＂ms＂ and the fertile restoration gene Msr. After three times recurrent backcrossing, a new genetic male sterile line of Chinese cabbage with 100% of male sterile plants and 100% of male sterility, BGMS-3, was bred. The new male sterile line was applied to develop hybrid combinations, and then one excellent combination of C3 was selected from six combinations.