热环境及主导管结扎后SD大鼠下颌下腺内Integrinα_6 β_1 及CD90.1阳性细胞增殖的比较

Proliferation of integrinα_6 β_1 and CD90. 1 positive cells in submandibular gland after heat acclimation or duct ligation in SD rats

目的:比较热环境与主导管结扎后SD大鼠下颌下腺内Integrinα6β1、CD90.1阳性细胞的增殖能力。方法:15只SD大鼠平均随机分为3组(n=5)。加热组大鼠在(34.5±1)℃环境饲养48 h;结扎组下颌下腺主导管结扎6 d;正常组予正常环境饲养。实验终点时摘除双侧下颌下腺腺体行组织学观察;流式细胞术分析腺体中Integrinα6β1、c-Kit、CD90.1阳性细胞百分比。结果:加热组下颌下腺体积重量最大,结扎组最小。流式细胞检测加热组、结扎组、正常组腺体内Integrinα6β1阳性细胞数分别为(26.2±2.4)%、(16.9±2.6)%、(10.6±0.7)%(P<0.05),CD90.1阳性细胞分别为(33.0±5.4)%、(25.0±4.1)%、(18.1±3.4)%(P<0.05),c-Kit阳性细胞分别为(13.4±3.4)%、(13.8±3.0)%、(8.5±3.1)%,加热组与结扎组无明显差异(P>0.05),但均明显高于正常组(P<0.05)。结论:热环境比主导管结扎更能有效刺激下颌下腺内Integrinα6β1和CD90.1阳性细胞的增殖,但对刺激c-Kit阳性细胞增殖没有明显差异。

Objective： To study the proliferation ability of integrinα6β1 and CD90.1 positive cells in the submandibular gland of SD rats after main duct ligation or heat acclimation. Methods： 15 SD rats were averaged into 3 groups（ n = 5 ） randomly. In heat acclima- tion group（ HG）, the rats were placed in an ambient temperature of （34.5 -1 ） % for 48 h. In duct ligation group（ LG）, the main ducts of submandibular gland of the rats were ligated for 6 days. In control group （NG） , the rats were fed in normal environment. The morphology of the glands was histologically observed, the percentage of integrinα6β1 , CD90.1 and c-Kit positive cells was examined by Flow Cytometry（FCM）. Results： The glands volume was the smallest in LG and the biggest in HG. FCM analysis demonstrated that the percentage of integrinα6β1 positive cells in the HG, LG and NG were （26.2 ± 2.4） %, （ 16.9 ± 2.6） % and （ 10.6± 0.7 ） % , CD90.1 positive cells were （33.0±5.4）%, （25.0±4.1）% and （18.1±3.4）% （P 〈0.05）, c-Kit positive cells were （ 13.4 ± 3.4 ）%, （13.8 ± 3. O）% and （8.5 ± 3.1 ）%, respectively. The percentage of c-Kit positive cells had no statistic difference between HG and LG（ P 〉 O. 05 ）, in HG and LG were higher than in NG（ P 〈 0.05 ）. Conclusion： The heat-condition may be more effective to induce the proliferation of integrinα6β1 and CD90. 1 positive cells than duct ligation, and similar to duct ligation in proliferation stimulation c-Kit positive cells.