昆明小鼠iPS细胞传代培养方法的优化研究

Optimization of the Culture Condition for Mouse iPS Cells

目的:探索不同胰蛋白酶序贯传代方法对小鼠诱导多潜能干(iPS)细胞培养的影响。方法:利用不同胰蛋白酶序贯方法消化小鼠iPS细胞(实验组),与传统的单一胰蛋白酶传代方法(对照组)进行比较,利用流式细胞学计数技术比较2组消化后的细胞中iPS细胞的比例。结果:iPS细胞所占比例在实验组、对照组分别为60.25%、47.06%,有显著性差异(P<0.01)。结论:用不同胰蛋白酶序贯消化方法对小鼠iPS细胞进行传代培养,可提高小鼠iPS细胞的纯度,有利于小鼠iPS细胞的扩增培养。

Objective： To study the effect of different trypsin sequential method on the culture of mice-derived induced pluripotent stem （iPS） cell. Methods： Mouse iPS cells were digested by the different trypsin sequential method. The percentage of iPS cells was determined by flow cytometry and compared with that treated by the single trypsin method. Result： The percentage of iPS cells cultured with different trypsin sequential method is higher than that with single trypsin method. Conclusion： The different trypsin sequential method could improve the purity of iPS cells. Mouse iPS cells culture could be optimized in this way.