mTOR-Notch通路在人乳腺癌组织中的验证

Correlative Activation of mTOR and Notch Pathways in Human Breast Cancer.

目的在人乳腺癌组织中验证mTOR与Notch通路的相关性。方法收集33例人乳腺癌组织样本通过Western blot检测mTOR下游信号pS6与Notch下游因子Hes1表达水平;57例人乳腺癌组织样本标本进行免疫组化染色,5例乳腺腺瘤及腺病标本作为对照,检测mTOR下游信号蛋白pS6与Notch的表达水平。将Western blot结果和免疫组化染色结果定量后采用Stata 9.2软件统计分析二者的相关性。结果在所有的有效样本中(Western blot 30例,免疫组化57例),mTOR与Notch的表达水平正相关。结论乳腺癌中mTOR和Notch通路共同活化,两条通路可能存在相关性。

Objective To examine the correlation of mTOR and Notch in human breast cancer tissues.Methods Western blot of pS6,the direct target of mTOR and HES1,the canonical target of Notch was done on 33 human breast cancer tissues.Immunohistochemistry （IHC） staining of pS6 and Notch1 was done on 57 human breast cancer tissues.The results were then quantified and statistically analyzed to reveal the correlation of mTOR and NOTCH activation by Stata 9.2.Results mTOR and Notch had significant relevance in the breast cancer samples examined （30 samples using Western blot and 57 samples using IHC）.Conclusion The coactivation of mTOR and Notch signaling pathways suggests the correlation of the two pathways in breast cancer.