氟暴露患者外周血中MCM3的表达及肝肾功能变化

Expression of minichromosome maintenance 3 from the peripheral blood of fluorosis patients and the liver and renal function

背景:前期研究发现MCM3与氟中毒相关,但其在氟中毒早期暴露者中的表达情况尚不清楚。目的:分析氟暴露患者及对照人群外周血中MCM3 mRNA的表达量。方法:选取饮水型氟中毒轻度患者(暴露组)、对照人群(非暴露组)各11例,采用SYBRGreen1嵌合荧光法实时定量PCR检测外周血单个核细胞中MCM3 mRNA的表达,同时测定两组肝肾功能指标。结果与结论:暴露组及非暴露组MCM3 mRNA表达量分别为0.573 60±0.102 59、0.550 0±0.171 81,两组差异无显著性意义(P>0.05)。所测的肝肾功能指标在两组中差异无显著性意义。结果显示轻度氟暴露对患者外周血单个核细胞中MCM3 mRNA表达无显著影响。氟对暴露者肝、肾功能的影响需要选取更多的指标综合分析。

BACKGROUND： Previous studies have shown that minichromosome maintenance 3 is related with fluorosis, but the expression of minichromosome maintenance 3 in fluorosis patients is not clear yet. OBJECTIVE： To analyze the mRNA expression level of minichromosome maintenance 3 in peripheral blood from patients exposed to fluoride and normal controls. METHODS： Eleven patients with mild fluorosis by drinking water （exposure group） and 11 cases of control （non-exposure group） were selected for research. SYBRGreenl real-time quantitative PCR was used to determine the rnRNA expression of minichromosome maintenance 3 in peripheral blood mononuclear cells, and the liver and renal function indicators were detected. RESULTS AND CONCLUSION： mRNA expressions of minichromosome maintenance 3 in the exposure group and non-exposure group were （0.573 60＋0.102 59） and （0.550 0＋0.171 81）, respectively, and there was no significant difference between two groups （P 〉 0.05）. There were no significant differences in the liver and renal function indicators between two groups. The results indicate that mild fluorosis has no significant effect on mRNA expression of minichromosome maintenance 3 in the peripheral blood mononuclear cells. More indicators are needed to compressively analyze the effect of fluoride on the liver and renal functions.