酶联免疫法测定去氨加压素血药浓度及其药动学

Study on the pharmacokinetics and ELISA method used for determining the blood concentration of desmopressin

目的:建立用酶联免疫(ELISA)法测定去氨加压素血药浓度的快速、简便方法,并研究其药动学。方法:用人去氨加压素(dDAVP)ELISA试剂盒对8例受试者给药后各时间段的血样进行血管加压素含量测定,用SPSS 18.0对检测平均值作药时曲线图并计算药动学相关参数。结果:ELISA法测定去氨加压素标准曲线回归方程为:Y=92.364 X+9.655 5,R2=0.998 1,平均回收率为100.96%;t1/2=(81.1±10.9)min;Cmax=(175.1±8.8)pg·mL-1;AUC0→200=(18 415±964)pg·h·mL-1;ClT=(52.8±2.6)mL·min-1。结论:ELISA法用于测定去氨加压素血药浓度及药动学研究的方法快速方便,结果稳定可靠。

To develop a simple and rapid method for determining the concentration of desmopressin and study its pharmacokinetices. METHODS Blood samples of 8 patients collected at different time after administration were determined using ELISA kit. The AUC and pharmacokinetics parameters were calcultated by SPSS 18.0 software. RESULTS The calcula tion curve was Y= 92. 364X＋ 9. 655 5,R2 = 0. 998 1. Recovery rate was 100. 96%. Half life time was （81.1± 10. 9）rain and Cm.x was （175.1± 8. 8）pg.mL 1. AUC2.. was （18 415± 964）pg-h-mL i. CIT was （52. 8± 2. 6）mL.min-1. CONCLUSION The developed method to study the concentration and pharmaeokinetics of desmopressin using ELISA was fast and reliable.