青牛胆总黄酮的对比提取研究

Extraction of Total Flavonoids from Tinospora sagittata of Tongdao

采用分光光度法对青牛胆块根中总黄酮进行超声波法、浸提法和微波法比较提取研究。以总黄酮提取率为考察对象,研究提取温度、乙醇体积分数、料液比及提取时间等因素对青牛胆块根中总黄酮提取率的影响。结果表明:最佳提取方法为浸提法,最佳提取条件:乙醇体积分数60%,提取温度90℃,料液比1:30,提取时间4h,总黄酮提取率6.294 mg/g。

In this study, three extraction methods, namely ultrasonication, lixiviation and microwave, were compared for the extraction of total flavonoids from Tinospora sagittata root by UV spectrometer. The effects of extraction temperature, concentration of aqueous ethanol,ratio of material-solvent, and extraction time on the extraction yield of total flavonoids were investigated. The results showed that the best extraction method was the lixiviation method, the optimal extraction conditions was determined to be：extraction temperature was 90 ~C, concentration of aqueous ethanol was 60%, ratio of material-solvent was 1： 30, extraction time was 4 h. Using the optimal extraction condition,the extraction yield of total fla- vonoids was 6. 294 mg/g.