响应面法优化紫甘薯花色苷水解条件

Optimization of Conditions for Anthocyanin Hydrolysis from Purple Sweet Potato by Response Surface Methodology

使用响应面法优化了紫甘薯花色苷的水解条件。使用高效液相色谱-多波长检测器测定花青素含量。采用3因素3水平Box-Behnken中心组合设计,优化了水解时间、盐酸浓度和水解温度对矢车菊素和芍药素峰面积之和的综合影响。结果显示,紫甘薯花色苷的最佳水浴水解条件为:盐酸浓度4.54 mol/L,水浴温度100℃,水解时间45.8 min。在最优条件下进行验证试验,响应值为理论值的96.5%,说明该响应面法优化方法可行。

The hydrolysis conditions for anthoeyanin from purple sweet potato （PSP） was optimized with response surface methodology （RSM）. The aglyeon forms of the anthocyanins were quantified using high performance liquid chromatogra- phy with multi wavelength detector. The combined effects of three independent variables：HC1 concentration,hydrolysis temperature, and hydrolysis time were optimized with a 3-factors-3-1evels Box-Behnken central composition experiments design. The results showed that the maximum yield of anthocyanidins was obtained with the hydrolysis using a HCI con- centration of 4.54 mol/L,a water bath temperature of 100 ℃ ,and a duration of 45.8 min. The reliability of the method was confirmed by validation tests performed under optimal condition and the sum of obtained peak areas of anthocyani- dins is 96.5% of the predicted value,which demonstrated the feasibility of the RSM optimization.