高效液相色谱检测青海湖嗜盐菌胞内积聚的相溶物质四氢嘧啶

Detection of intracellular compatible solute ectoine in halophilic bacteria from Qinghai Lake by high performance liquid chromatography

从20份青海湖水样中,分离获得了35株中度嗜盐菌株.耐盐梯度实验表明:水体中以中度嗜盐菌为主,约占65.7%;弱嗜盐菌次之,约占25.7%;非嗜盐菌与耐盐菌相对较少,约占8.5%.采用80%乙醇法抽提中度嗜盐菌胞内的Ectoine,利用TLC和HPLC分析,结果表明:青海湖中度嗜盐菌中,大多数胞内积聚相溶物质Ectoine;在最适生长盐度下,中度嗜盐菌QHL1、QHL5、QHL10、QHL26和QHL28的胞内Ectoine含量分别为258.6μg·mL-1、325.47μg·mL-1、208.8μg·mL-1、292.31μg·mL-1和295.13μg·mL-1,以此初步获得5株具有Ectoine高产潜力的野生菌株.

In this study, we isolated 35 bacterial strains in 20 water samples from Qinghai Lake. The results from salt resistant gradient experiments showed that moderate activity halophiles accounted for about 65.7%, while weak activity halophiles were about 25.7%. Nonhalophile and halotolerant were relatively low, only about 8.5%. The accumulation of ectoine in moderate activity halophilic bacteria was analyzed by TLC and HPLC, and the results revealed that compatible solute ectoine accumulated in most moderate halophiles. Under the optimum growth salinity, the amount of ectoine in QHL1, QHL5, QHL10, QHL26 and QHL28 reached 258.60, 325.47, 208.8, 292.31, 295.13 μg·mL^-1respectively. Finally, five wild strains with high ectoine production capability were obtained.