PCR-DGGE技术在废水生物处理中的应用研究

PCR-DGGE Technique in Biological Wastewater Treatment Application

聚合酶链式反应-变形梯度凝胶电泳(PCR-DGGE)作为一种分子指纹图谱能够较准确地反应污水处理过程中微生物群落结构多样性及其动态变化而广泛应用于废水生物处理技术的研究中。PCR-DGGE技术不仅能对样品中微生物群落结构多样性及种群演替进行分析,还能用于污水处理中优势菌群的分离与鉴定,克服了传统方法费时费力、培养条件苛刻等局限性,进而运用该技术构建高效的污染物降解工程菌,提高难降解有机物的去除效率,调节实际工艺参数,提高污水处理效率。

Polymerase Chain Reaction - Deformation Gradient Gel Electrophoresis （ PCR - DGGE） as a molecular finger- print can more accurately reflect the sewage treatment process microbial community structure and dynamics and diversity are wide- ly used in biological wastewater treatment. PCR - DGGE technique can not only diversity of microbial community structure in the sample and population succession analysis, but also for sewage treatment isolation and identification of advantage bacteria to over- come the traditional method is time consuming and laborious, culture conditions such as harsh limitations Furthermore the use of the technology to build efficient degradation of pollutants engineered bacteria to improve the efficiency of refractory organics remov- al, adjust the actual process parameters to improve the efficiency of wastewater treatment.