摘要
目的从细胞分子水平研究电压门控型钾通道亚型Kv1.2、Kv3.4在15-酮基二十碳四烯酸(15-ketoeicosatetretraenoic acid,15-KETE)致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD大鼠肺动脉平滑肌细胞(pulmonary arterial smooth cells,PASMCs),使用RT-PCR和Western blot技术观察15-KETE对大鼠PASMCs上Kv1.2、Kv3.4通道表达的影响。结果 (1)15-KETE下调大鼠PASMCs膜上Kv1.2通道mRNA及蛋白质的表达;(2)15-KETE下调大鼠PASMCs膜上Kv3.4通道mRNA及蛋白质的表达。结论 Kv1.2、Kv3.4通道参与由15-KETE引起的缺氧肺动脉收缩(hypoxic pulmonary vasoconstriction,HPV)。
Objective To observe the effect of Kv1.2、Kv3.4 channels in rat pulmonary arterial smooth cells on the process of pulmonary vasoconstriction induced by 15-KETE.Method Western blot and PCR were used to identify the effect of 15-KETE on expression of Kv1.2、Kv3.4 channels in cultured rat pulmonary arterial smooth muscle cells（PASMCs）.Result（1） The expressions of Kv1.2 mRNA and protein in rat PASMCs were significantly down-regulated by 15-KETE.（2） The expressions of Kv3.4 mRNA and protein in rat PASMCs were significantly down-regulated by 15-KETE.Conclusion Kv1.2、Kv3.4 channels are involved in vasoconstriction induced by 15-KETE in rat PASMCs.
出处
《实验动物科学》
2013年第3期19-24,共6页
Laboratory Animal Science
基金
黑龙江省科技攻关项目(No.PC09S04)
