小鼠早期胚胎不同移植方法的建立

The Establishment of Method on Early Mouse Embryo Transplantation

目的建立小鼠胚胎移植技术,探索小鼠胚胎输卵管伞部移植、输卵管剪口移植和子宫移植技术要点,为进一步将该项技术应用到生物净化等领域提供参考数据。方法对4周龄C57BL/6雌性小鼠采用腹腔注射7.5 IUPMSG(孕马血清促性腺激素),48 h后腹腔注射7.5 IU HCG(人绒毛膜促性腺激素)进行超数排卵处理,并与C57BL/6雄鼠合笼,分别在见栓0.5 d、1.5 d和3.5 d取出相应胚胎,对所得胚胎进行筛选,将质量较好的胚胎选出,并将相应胚胎阶段(单细胞、2细胞、囊胚)移入同期发情并经KM结扎雄鼠交配刺激后见栓的KM假孕小鼠输卵管或子宫内,待妊娠生产后得到移植小鼠。结果对88只4周龄雌性C57BL/6小鼠进行超数排卵处理,见栓62只,共取得单细胞胚胎510枚、2细胞期胚胎400枚、囊胚35枚,采用输卵管伞部移植、输卵管剪口和子宫移植,共移植假孕母鼠43只,妊娠生产39只,产仔361只。结论输卵管伞部移植与输卵管剪口移植比较无显著差异,输卵管伞部移植对动物伤害较小,但对术者要求较高;输卵管剪口移植操作较快捷,但对动物损伤较大;子宫移植的妊娠效果最好,处于这一阶段的胚胎极易着床。

Objective Establish mouse superovulation and embryo transfer technology,in order to further use the technology to control their offspring,and gradually will these technologies applied to other animals,so as to speed up its breed improvement,improve the utilization rate of good varieties and individual.Method Of 4-week-old C57BL/6 female mouse by intraperitoneal injection 7.5 IU PMSG（pregnant mare serum gonadotropin） 48 hours after intraperitoneal injection of superovulation 7.5 IU HCG（human chorionic gonadotropin） and C57BL/6 male rats mated,see bolt 0.5 days,1.5 days and 3.5 days,remove the corresponding embryo,the resulting embryo screening,elect better quality embryos and embryonic stages（single cells,2 cells capsule fallopian tube or uterus KM pseudopregnant mice mouse embryo） into estrus synchronization see bolt after KM ligation male rats mating stimulus be in production pregnancy after transplantation in mouse.Result Superovulation 88 C57BL/6 mice,see bolt 62,a total of 510 single-cell embryos,400 2-cell stage embryos,blastocysts 35,the ministry of tubal transplantation,tubal notches,uterine transplant transplant pseudopregnant female mice 43,pregnancy producing 39 farrowing 361.Conclusion Tubal fimbria transplantation compared with tubal Jiankou transplant was no significant difference tubal transplantation hurt small animals,but for patients who require a higher;fallopian tube the Jiankou transplant operation is relatively quick,but the larger animal injury;uterine transplant the pregnancy best effect at this stage of embryo easily implantation.