利用胺磺灵诱变西瓜四倍体研究

Study on Mutugenesis of Tetraploid Watermelon by Using Oryzalin

为了给无籽西瓜的选育提供优质亲本材料,以小果型西瓜二倍体自交系A8为试材,采用胺磺灵对幼苗生长点进行剥滴处理,设计不同浓度和处理天数,进行四倍体诱导,并进行形态学鉴定、流式细胞仪分析鉴定、保卫细胞叶绿体计数法鉴定,经3代种植观察和筛选,获得了稳定的四倍体材料。结果表明:不同处理均可诱变产生该材料的四倍体,其中最有效的处理是在播种后第8天剥除心叶,再用100mg/L胺磺灵连续处理裸露的生长点6d,幼苗成活率达100%,最终诱变率达30.59%。

In order to provide breeding high-quality parent material for seedless watermelon, taking small-type diploid watermelon line A8 as material, tetraploid plants of watermelon were induced by treating the growing points of seedlings with different concentration Oryzalin and lasting different treating time. Autotetraploid plants were selected and identified basing on morphology, flow cytometry, hybridization and characterization. Then observed a stable tetraploid material after three quarters; continuous cultivation,observation and filtering. The results showed that different treatments could obtain tetraploid watermelons by processing with Oryzalin. The most effective way was extirpating interiorleaf at the 8th day after sowing,then treating the growing points of seedlings with 100 mg/L Oryzalin for 6 d. The treatmented seedlings could all survival and the induction frequency of tetraploid watermelon was 30. 59 %.