摘要
以蒙古栎促萌枝条的幼嫩茎段为外植体,研究了不同种类的基本培养基、不同浓度的外源激素及其配比对蒙古栎器官发生及植株再生的影响。结果表明:蒙古栎促萌枝条的幼嫩茎段可通过器官发生途径获得再生植株,最佳初代培养基为WPM+6-BA 0.5mg/L+2,4-D0.5mg/L,诱导率为76.7%;最佳不定芽增殖培养基WPM+6-BA 2.0mg/L+NAA 0.5mg/L,增殖系数为3.5;最佳生根培养基为1/2MS+NAA 0.5mg/L+IBA 0.1mg/L,生根率达76.7%。
Taking the forced shoots of Quercus mongolica as a source of initial explants, the effects of different types of media and different concentrations of plant growth regulators and their ratios on organogenesis and plantlet regeneration of Quercus mongolica were studied. The results showed that regenerated plants could be obtained from forced shoots of Quercus mongolica via organogenesis. The best initial medium was WPM＋6-BA 0. 5 rag/L＋2, 4-D 0. 5 rag/L, the induction rate was 76.7%. The best adventitious bud proliferation medium was WPM＋6-BA 2. 0 mg/L＋NAA 0. 5 mg/L and the highest proliferation factor was 3. 5. The best rooting medium was 1/2MS＋NAA 0. 5 mg/L＋IBA 0. 1 mg/L and the rooting rate was up to 76. 7 %.
出处
《北方园艺》
CAS
北大核心
2013年第19期102-105,共4页
Northern Horticulture
关键词
蒙古栎
促萌
组织培养
植株再生
Quercus mongolica
forced shoot
tissue culture
plant regeneration
