摘要
以2个高感白粉病的甜瓜品种"K413"、"JS"和3个高抗品种"H70"、"AN"、"H74"为试材,采用PCR方法,对第Ⅴ连锁群的Pm-AN片段进行克隆分析。结果表明:从"K413"、"JS"、"H70"、"AN"和"H74"中克隆出Pm-AN-N/C 2个片段分别得到830bp和610bp 2个碱基,分别编码氨基酸286个和192个,同源性分别为99.74%和94.00%。cDNA扩增结果表明,5个甜瓜品种分别出现5种不同的带型,在"JS"、"H70"、"AN"中还出现了2种不同的带型,而且所有带型都有一定的差异。
Taking two high sensitive melon powdery mildew varieties ＇K413＇, ＇JS＇ and three high resistant varieties ＇ H70＇,＇AN＇, ＇ H74＇ as test materials,using PCR method, part V linkage group Pro-AN was cloned and analyzed. The results showed that the cloned Pm-AN-N/C two pieces were 830 bp and 610 bp respectively from ＇K413＇, ＇JS＇,＇ H70＇, ＇AN＇ and ＇H74＇,encoding amino acids 286 and 192 respectively,homology of 99. 74% and 94.00% respectively, cDNA amplification results showed that the five melon varieties appeared five different band type,respectively,appearing in two different type from ＇JS＇, ＇H70＇, ＇AN＇ ,and all model had difference.
出处
《北方园艺》
CAS
北大核心
2013年第19期110-113,共4页
Northern Horticulture
基金
新疆自治区高技术研究发展资助项目(201111120)
国家自然科学基金资助项目(31060148)
关键词
甜瓜
抗白粉病基因
基因克隆
序列分析
melon
powdery mildew candidate gene
gene cloning
sequence analysis
