乙肝五项两种主要模式与HBV-DNA载量关系研究

Results and Analysis of HBV-DNA and Two Main Serological Markers of Hepatitis B about HBV Infection

目的：研究乙型肝炎患者乙肝五项两种主要模式与HBV-DNA栽量之间的关系。方法：采用ELISA法检测乙肝五项血清标志物，FQ-PCR法检测HBV-DNA载量。结果：随着HBV-DNA拷贝数的增加，大三阳所占比率随之升高，小三阳则反之。当HBV-DNA含量为105～107IU／mL时，大三阳HBV-DNA栽量明显高于小三阳（P〈0．05），且男性青少年中HBV-DNA复制要比中老年活跃（P〈0．01）。结论：HBeAg是反映HBV复制活跃程度的可靠指标，联合检测HBV-DNA栽量能更好地为临床诊断HBV提供准确可靠的参数。

Objective：To investigate the correlation between the two main serological markers and the contents of HBV-DNA in he- patics B patients. Methods. ELISA was applied to determine HBsAg,anti- HBs , HBeAg,anti - HBe and anti-HBc IgM. FQ-PCR was used to measure the contents of HBV-DNA in serum. Results ： The percents of 1,3,5 modes were much higher than 1,4,5 mod- els when HBV-DNA replicates actively. The contents of HBV-DNA in 1,3,5 models were higher than 1,4,5 models between 105 107 IU/mL,and the contents of HBV-DNA of the younger were higher than the older in males. Conclusion： HBeAg was an impor- tant marker of viral replication,and joint detection of Hepatitis B serological markers and HBV-DNA can provide accurate and relia- ble data for clinical diagnosis.