高效液相色谱法测定妇康宁片中阿魏酸的含量

The content determination of ferulic acid in Fukangning tablets by HPLC

目的建立高效液相色谱法测定妇康宁片中阿魏酸含量的方法。方法色谱柱为SinoChrom ODS-BPC18柱（4．6mm×250mm，5μm）；流动相为乙腈-0．1％磷酸（22：78）；流速：1．0ml/min；检测波长：316nm；柱温：25℃。结果阿魏酸在0．0014～0．0280mg／ml范围内呈良好的线性关系（r=0．9998），平均回收率为99．2％，RSD=1．0％（n=6）。结论本方法操作简单、快速。可用于测定妇康宁片中阿魏酸的含量。

Objective To establish a method for the content determination of ferulic acid in Fukangning tablets by HPLC. Methods SinoChrom ODS-BP C18 column （4.6 mm×250 mm,5 μm） was used;the mobile phase consisted of acetonitrile-0.1% phosphate acid （22：78） and the flow rate was 1.0 ml/min.The detection wavelength was 316 nm and the column temperature was at 25℃. Results Ferulic acid showed a good linear relationship （r=0.9998） in the range of 0.0014-0.0280 mg/ml with the addition recovery rate of 99.2% and RSD of 1.0%（n=6）. Conclusion The method is simple and accurate with good repetition and high precision,which was suitable for the determination of ferulic acid in Fukangning tablets.