枇杷清热养颜颗粒两种主要活性成分的质量控制研究

Quality control research of two kinds of main active components in Pipaqingreyangyan particle

目的建立高效液相色谱法同时测定枇杷清热养颜颗粒中绿原酸和黄芩苷的含量。方法Agilent SB—C18柱，流动相：甲醇-0．2％磷酸水溶液（52：48）；流速：1．0ml／min；检测波长：324nm；柱温：室温。结果绿原酸线性范围为0．86～41．28mg／L，平均回收率为100．14％（RSD=1．06％），回归方程为Y=4028．2X-13．106，r=0．9999；黄芩苷在0．7841．13mg／L范围内呈良好的线性关系，回归方程为：Y=3430．2X＋119．99（r=0．9992），平均回收率为100．06％（RSD=1．09％）。结论此方法简便可靠，结果稳定，重复性好，可用于枇杷清热养颜颗粒中绿原酸和黄芩苷的含量测定。

Objective To determine the content of chiorogenic acid and baicaiin simultaneously in Pipaqingreyangyan particle by high performance liquid chromatography. Methods Agilent SB-C18 column was used;the mobile phase was methanol-0.2% phosphoric acid solution （52：48）;the velocity was 1.0 ml/min;the detection wavelength was 324 nm and the column temperature was room temperature. Results The linear range of chlorogenic acid was from 0.86 to 41.28 mg/L,the average recovery rate was 100.14%（RSD=1.06%）,the regression equation was Y=4028.2X-13.106,r= 0.9999.Baicalin appeared good linear relationship in the range of 0.78-41.13 mg/L,the regression equation was Y= 3430.2X＋119.99 （r=0.9992）,the average recovery rate was 100.06%（RSD=1.09%）. Conclusion This method is simple and reliable,and it has stable result and good repeatability,which can be used for the content determination of chlorogenic acid and baicalin in Pipaqingreyangyan particle.