磷脂转运蛋白和巨噬细胞移动抑制因子在支气管肺发育不良小鼠血清中表达及意义

Expression and significance of PLTP and MIF in mice with bronchopulmonary dysplasia

目的探讨支气管肺发育不良(BPD)小鼠血清巨噬细胞移动抑制因子(MIF)及磷脂转运蛋白(PLTP)水平变化及意义。方法 96只4日龄小鼠,随机分为氧气组和空气组。氧气组置于氧浓度为65%的氧箱中,空气组暴露于空气中。分别于实验的第7、14、21、28天时每组随机选取12只小鼠,留取血标本和肺脏;通过酶联免疫法(ELISA)测定血液MIF及PLTP水平,HE染色观察肺组织形态变化。结果氧气组小鼠肺组织病理表现为肺实质增厚、肺纤维化明显,放射性肺泡计数明显低于空气组,差异有统计学意义(P<0.01)。空气组小鼠PLTP水平在第7、14、21天逐渐升高,第28天开始降低;而氧气组小鼠在第7、14天升高,第21、28天降低。空气组小鼠MIF水平无明显改变;氧气组小鼠在第7、14、21天逐渐升高,第28天下降。结论 MIF及PLTP可能是BPD的生物学标志。

Objective To study the expression and significance of phospholipid transfer protein （PLTP） and macrophage migration inhibitory factor （MIF） in mice with bronchopulmonary dysplasia （BPD）. Methods Ninety-six 4-day-old mice were randomly divided into oxygen group and air group. Mice in oxygen group were exposed to a FiO2 of 65%, and mice in air group were exposed to air. On day 7, 14, 21 and 28, blood and lung tissue samples from 12 randomly selected mice in each group were obtained. The serum levels of MIF and PLTP were measured by ELISA assay. The morphological changes of lung tissue were ob- served with HE staining. Results The mice in oxygen group showed thickened lung parenchyma and obvious pulmonary fibrosis. The radioactive alveolar count was significantly lower in oxygen group than that in air group （P〈0.01）. PLTP level in air group was increased gradually from day 7 to day 21, and began to decrease on day 28. PLTP level in oxygen group was increased from day 7 to day 14, and decreased on day 21 and day 28. MIF level in air group did not change during the experiment. MIF level in oxygen group was significantly increased from day 7 to day 21, and began to decrease on day 28. Conclusions MIF and PLTP may begood biomarkers for the diagnosis of BPD.