辽宁省冬季温室内越冬粉虱伪蛹的种类鉴定及烟粉虱体内番茄黄化曲叶病毒的检测

Species identification of over-wintering whitefly pseudo-pupae and detection of the infection status of tomato yellow leaf curl virus in Bemisia tabaci in winter greenhouses in Liaoning,northeastern China

为了揭示辽宁省冬季温室内越冬粉虱伪蛹的种类及烟粉虱Bemisia tabaci(Gennadius)携带番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)情况,于2012年1月份在辽宁省不同县市区的温室作物上采集了17份粉虱伪蛹样品(每样品含30头粉虱伪蛹),镜检鉴别粉虱种类并利用mtCOI基因对烟粉虱生物型进行了鉴定;检测了烟粉虱携带TYLCV情况并对其PCR扩增产物进行了测序分析。结果表明:辽宁省冬季温室内存在越冬温室白粉虱Trialeurodes vaporariorum(Westwood)与烟粉虱。17份粉虱样品中,11份样品为烟粉虱样品,6份样品为温室白粉虱和烟粉虱混合样品。混合样品中,温室白粉虱仅在锦州凌海(LH)样品中占优势。17份烟粉虱样品(包括混合样品)中,仅有4份样品为B型与Q型混合样品,其他13份样品烟粉虱生物型均为Q型。17份烟粉虱样品中有3份Q型烟粉虱样品检测到TYLCV,系统树分析进一步证实该病毒是TYLCV。调查结果为辽宁省粉虱与TYLCV的早期测报和防控提供了科学依据。

To identify the species of over-wintering whitefly pseudo-pupae and detect the infection status of tomato yellow leaf curl virus （TYLCV） in Bemisia tabaci （Gennadius） in winter greenhouses in Liaoning, northeastern China, 17 samples of whitefly pseudo-pupae were collected from different counties in the province in January, 2012. The whitefly species was identified under microscope based on morphological characteristics and the biotype of B. tabaci was determined using mitochondrial cytochrome oxidase I （mtCOI） gene. Then TYLCV in the whitefly vector, B. tabaci was checked using specific primers and the product was sequenced and analyzed. The results showed that there were two whitefly species, i. e., Trialeurodes vaporariorum （Westwood） and B. tabaci, over-wintering in winter greenhouses in the province. Of the 17 whitefly samples, 11 were B. tabaci B-biotype samples while the other 6 were mixtures of two whiteflies （ biotypes B and Q）. In addition, T. vaporariorum was dominated in only one mixture in Linghai, Jinzhou （code LH）. Of the 17 B. tabaci samples including the mixtures, only 4 samples were the mixture of biotypes B and Q, while the other 13 were Q-biotype samples. TYLCV was only detected in 3 Q-biotype samples. Phylogenetic analysis also reealed that the ＂titus was TYLCV. These results provide scientific basis for the forecast, prevention and control of the whiteflies and TYLCV in Liaoning, China.