植物根围外来微生物定殖的检测方法

Detection of introduced microorganisms to rhizosphere

检测植物根围微生物定殖情况的方法分为可培养的微生物和不能培养的微生物两种 .传统做法是用培养方法检测投入到根围的微生物 ;而现在逐渐注重研究对于不可培养土壤微生物定殖的检测 .综合检测方法有四种 :天然抗生素抗性的标记 ;外来基因标记 ;DNA和 RNA探针以及免疫学方法 .前两种方法往往结合在一起应用于可培养的根围外来微生物的检测 ;而后二者可用于检测可培养的或不可培养的根围微生物包括活细胞或死细胞 .根围不能培养的微生物已逐渐由于土壤

The microorganisms could be detected in rhizospere may be divided into two groups, the culturable and non culturable. The culturable group could be detected by spontaneous antibiotic resistance; marker genes; DNA or RNA probe; and Immunofluorescent colony staining. But the first two methods are often combined to be used for culturable group. The second two methods are used to explore the colonization of culturable or non culturable group including dead cells and live cells in rhizosphere. Lastly, the bigger progress in molecular microbial ecology in which non culturable soil mioroorgamisms can be detected by genomic DNA libraries which can be used to explore soil microbial diversity including the colonzation of introduced microbes to rhizosphere.