核素标记叶酸偶联白蛋白纳米微球对人卵巢癌细胞生长的影响

Effect of ^(188)Re-labeled folate coupling with magnetic albumin nanoparticles on growth of SKOV3 ovarian cancer cells in vitro

目的观察核素标记叶酸靶向白蛋白纳米微球对人卵巢癌细胞生长的影响。方法将体外培养的SKOV3人卵巢癌细胞分为八组:阴性对照组(只加RPMI-1640培养液),单纯化疗组(叶酸偶联载药白蛋白磁性纳米微球,不加磁场),单纯放疗组[188铼(188 Re)标记的叶酸偶联白蛋白磁性纳米微球,不加磁场],单纯热疗组(叶酸偶联白蛋白磁性纳米微球,加磁场),化疗联合放疗组(188 Re标记的叶酸偶联载药白蛋白磁性纳米微球,不加磁场),化疗联合热疗组(叶酸偶联载药白蛋白磁性纳米微球,加磁场),放疗联合热疗组(188 Re标记的叶酸偶联白蛋白磁性纳米微球,加磁场)和热疗、化疗、放疗联合治疗组(联合治疗组,188 Re标记的叶酸偶联载药白蛋白磁性纳米微球,加磁场)。48h后MTT方法测定各组细胞增殖率,流式细胞术测定细胞凋亡率。结果阴性对照组对卵巢癌细胞增殖的抑制作用弱于其他各组,联合治疗组强于其他各组(P<0.05)。单纯热疗组、化疗联合放疗组、化疗联合热疗组、放疗联合热疗组和联合治疗组中细胞周期G1期前出现明显的亚二倍体凋亡峰;阴性对照组、单纯热疗组、化疗联合放疗组、化疗联合热疗组、放疗联合热疗组和联合治疗组的细胞凋亡率分别为0.08%、7.56%、17.14%、21.64%、33.94%和57.16%。结论磁感应热疗、化疗、核素靶向放疗的联合作用能有效抑制人卵巢癌细胞的生长。

Objective To investigate the effect of 188Re-labeled folate coupling with magnetic albumin nanoparticles on the growth of SKOV3 ovarian cancer cells in vitro. Methods SKOV3 cells cultured in vitro were divided into 8 groups of A（treated with RPMI-1640 medium as the control）, 13 （treated with folate coupling with dru^loaded magnetic albumin nanoparticles）, C （treated with 188Re-labeled folate coupling with magnetic albumin nanoparticles）, D （treated with folate coupling with magnetic albumin nanoparticles in magnetic field）, E（treated with 188 Re-labeled folate coupling with drug-loaded magnetic albumin nanoparticles）, F（treated with folate coupling with drug-loaded magnetic albumin nanoparticles in magnetic field）, G（treated with 188Re-labeled folate coupling with magnetic albumin nanoparticles in magnetic field） and H（treated with 188Re-labeled folate coupling with drug-loaded magnetic albumin nanopartieles in magnetic field）. The eel1 proliferation rate and cell apoptosis rate were measured by MTT assay and flow eytometry after 48 hours, respectively. Results The inhibitory effect on proliferation of SKOV3 cells was weaker in group A than that in the other groups,which was stronger in group H than that in the other groups（P^0. 05）. Obvious apoptotic hypodiploid peak appeared before G1 phase in groups of D, E, F, G and H. The apoptotie rates of SKOV3 cells in groups of A,D,E,F,G and H were 0. 08%,7. 56%,17.14%,21. 64%,33. 94% and 57.16% ,respectively. Conclusion A combination of magnetic induction hyperthermia, chemotherapy and radionuclide targeted radiotherapy can efficiently inhibit the growth of ovarian cancer cells.