结直肠腺瘤和结直肠癌患者外周血Tc17和 Treg 细胞的变化

The changes of circulating Tc17 and Treg cells in the patients with colorectal adenoma and the pa-tients with colorectal cancer

目的检测结直肠腺瘤和结直肠癌患者外周血中Tc17与Treg细胞比例的变化，并分析二者相关性。方法流式细胞术检测30例结直肠腺瘤、33例结直肠腺癌患者和20例健康对照者外周血中Tc17和Treg细胞比例；ELISA检测其血清中IL-17A和IL-23水平。结果结直肠腺瘤和结直肠癌患者外周血中Tc17和Treg 细胞比例及细胞因子IL-17 A和IL-23水平均高于对照组（ P＜0．05）。结直肠癌组Tc17细胞比例及细胞因子IL-17A和IL-23水平均高于结直肠腺瘤组（P＜0．05），而Treg细胞比例在两组间并无统计学意义（ P＞0．05）。基于疾病进展，结直肠腺瘤Ⅰ级和早期结直肠癌组Tc17细胞比例及细胞因子IL-17A和IL-23水平分别高于相应的结直肠腺瘤Ⅱ级和晚期结直肠癌组（P＜0．05），Treg 细胞比例变化与 Tc17细胞变化相反，但二者无相关性（r ＝-0．227， P＝0．073）。结论结直肠腺瘤和结直肠癌患者外周血Tc17和Treg细胞比例随着疾病进展呈相反的变化趋势，但二者并不存在相关性。

Objective To observe the changes of circulating Tc 17 and Treg cells in the patients with colorectal adenoma（CRA）and the patients with colorectal cancer （CRC）and to investigate the correla-tions between them.Methods Peripheral bloods were collected from 63 patients （30 CRA and 33 CRC） and 20 healthy subjects .The proportions of circulating Tc 17 and Treg cells in peripheral blood mononuclear cells （ PBMCs） were detected by flow cytometry .The serum levels of IL-17A and IL-23 were measured by ELISA.Results Compared with healthy controls , the proportions of circulating Tc 17 and Treg cells as well as the serum levels of IL-17 A and IL-23 in CRA and CRC groups were significantly up-regulated （ P〈0.05）.The proportions of circulating Tc17 cells and the serum levels of IL-17A and IL-23 in CRC group were significantly higher than those in CRA group （P〈0.05）, but there was no significant difference in the expressions of Treg cells between the two groups .As the disease progressed , the proportions of circulating Tc17 cells and the serum levels of IL-17A and IL-23 in CRAⅠgroup and the early CRC group were respec-tively higher than those in CRAⅡgroup and the advanced CRC group （P〈0.05）, while the expressions of Treg cells was just the opposite , and no correlation between the expressions of Tc 17 and Treg cells was found （r=-0.227, P=0.073）.Conclusion The changes in the percentage of Tc17 cells in peripheral blood are contrary to Treg cells in patients with CRA or CRC as the disease progresses , but there is no correlation be-tween them .