精子计数池深度对精子活力影响的实验研究

Influence of the depth of the sperm counting chamber on sperm motility

目的:调查精子计数池深度对精子活力检测结果的影响。方法:利用Filmetrics间隙测量仪精确测量4种不同深度的Geoffrey精子计数池,然后按照WHO手册第5版的要求利用瑞祺CFT-9201型精子质量检测分析系统分析36例精液标本的前向运动精子百分率(PR)、非前向运动精子百分率(NP)和总活动率(PR+NP),并进行比较分析。结果:4种精子计数池的深度分别为9.8、12.7、15.7和19.9μm,测得的PR分别为(44.00±11.63)%、(41.96±12.62)%、(40.86±11.71)%和(37.78±11.38)%,NP分别为(13.54±3.01)%、(14.13±2.94)%、(14.91±3.02)%和(16.53±2.77)%,总活动率分别为(57.53±11.06)%、(56.08±11.97)%、(55.78±11.55)%和(54.31±12.11)%。精子计数板深度与PR呈显著负相关(r=-0.993,P<0.05),与NP呈显著正相关(r=0.989,P<0.05),与活动率呈显著负相关(r=-0.978,P<0.05)。尽管精子总活动率在4种不同深度的计数池之间没有显著差异,但PR和NP在9.8μm深的计数池和19.9μm深的计数池之间均有显著性差异(P<0.05)。结论:精子计数池的深度对精子活力的影响不容忽视,不同深度计数池获得的结果差异将会给临床医生对患者做出正确的诊断(如弱精子症)和采取合适的治疗措施带来一定的负面影响。不同深度的精子计数池应有相应的精子活力正常参考值范围。

Objective ： To investigate the influence of the depth of the sperm counting chamber on sperm motility. Methods ： We measured the depths of 4 sperm counting chambers using the Filmetfics F20 Spectral Reflectance Thin-Film Measurement System. Then, according to the WHO5 manual, we analyzed 36 semen samples for the percentages of progressively motile sperm （PR） and non- progressively motile sperm （NP） and sperm motility （ PR ± NP） with the Ruiqi CFF-9201 computer-aided sperm analysis system, and compared the results of analysis. Results ： The depths of the 4 sperm counting chambers were 9.8, 12.7, 15.7 and 19.9 μm, re- spectively, and the obtained PR were （44.00 ± 11.63 ）, （41.96 ± 12.62 ）, （40.86 ±11.71 ） and （37.78± 11.38 ） %, NP （ 13.54 ±3.01）, （14.13 ±2.94）, （14.91 ±3.02） and （16.53±2.77）%, and sperm motility （57.53±11.06）, （56.08±11.97）, （55.78 ±11.55 ） and （54.31 ± 12.11 ） % from the 4 chambers, respectively. The depth of the sperm counting chamber was correla- ted negatively with PR （ r = - 0. 993, P 〈 0.05 ） and sperm motility （ r = - 0.978, P 〈 0.05 ）, but positively with NP （ r = 0. 989, P〈0.05）. There were statistically significant differences between the 9.8 μm and 19.9 μm deep chambers in PR and NP （P 〈0.05） though not in sperm motility among the 4 chambers of different depths. Conclusion ： The impact of the depth of the sperm counting chamber on sperm motility should not be ignored, for the deviation of the results from the chambers of different depths may lead clini- cians to incorrect diagnosis and consequently inappropriate therapeutic approaches. Different reference ranges of sperm motility need to be normalized in correspondence to the depths of sperm counting chambers.