摘要
目的:研究中国荷斯坦牛生长激素受体(GHR)基因F279Y位点突变对产奶量及乳成份的影响。方法:以232头中国荷斯坦牛为研究材料,参照DHI生产性能测定方法采集产奶量和乳成份数据;采用单链构象多态性(PCR-SSCP)技术并结合测序确定基因型;用最小二乘法进行关联性分析。结果:中国荷斯坦牛GHR基因F279Y位点A、T等位基因频率分别为0.68和0.32,实验群体极显著偏离Hardy-Weinberg平衡(P<0.01);AA型305 d产奶量显著高于AT型(P<0.05),AT型305 d乳脂量、305 d乳蛋白量和305 d乳糖量在数值上有优于AA型的趋势;因此,等位基因A为高产奶量的优势基因,等位基因T对乳成份具有正效应。结论:GHR基因F279Y突变可作为遗传标记应用于中国荷斯坦牛泌乳性状的标记辅助选择(MAS)育种。
Objective: To study the effect of the polymorphism F279Y of the growth hormone receptor(GHR) gene on milk yield and composition in Chinese Holstein cattle. Methods: Hundred thirty two Chinese Holstein cattle were selected as study materials, according to DHI production performance method to get the data of milk yield and composition; PCR- SSCP and sequencing method were used to detect the genotypes; least square method was used to acquire correlation analysis. Results: Chinese Holstein cattle F279Y of GHR gene loci A and T allele frequency were 0.68 and 0.32, respectively, the experimental group significantly deviated from Hardy Weinberg equilibrium ( P 〈 0.01 ) ;305 d milk yield of AA genotype was significantly higher than AT type ( P 〈0.05), 305 d milk fat yield, 305 d milk protein yield and 305 d lactose of AT type had better trend than those of AA type in numeric; Therefore, allele A was dominant gene of high milk yield, allele T has positive effect on milk composition. Conclusion: Mutation F279Y of GHR gene can be used as genetic markers in Chinese Holstein milk production traits of marker assisted selection (MAS) breeding.
出处
《中国应用生理学杂志》
CAS
CSCD
2013年第5期400-404,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金项目(30960164)
科技部星火计划项目(2008GA860014)
甘肃省重大科技专项(0801NKDP037)
甘肃省生物技术专项(GNSW-2009-08)