MALDI-TOF-MS结合WCX技术在垂体腺瘤诊断中的应用

MALDI-TOF-MS combined with WCX technique in diagnosing pituitary adenoma

目的:应用基质辅助增强激光解吸电离飞行时间质谱(MALDI-TOF-MS)结合WCX纳米磁珠技术,筛选垂体腺瘤血清生物标志物,并建立血清蛋白指纹分类决策树。方法:分析40例垂体腺瘤、60例健康成人的血清样本,找到差异蛋白峰,建立垂体腺瘤的诊断模型和分类决策树。结果:在实验与对照组间找到42个差异蛋白峰(P<0.01),以其中质荷比(m/z)为3382.0、4601.9、9191.2的3个蛋白峰建立垂体腺瘤的诊断模型。该模型对正常人群筛选垂体腺瘤的敏感性90.00%,特异性为88.30%;经双盲实验验证,该模型对垂体腺瘤诊断的敏感性为88.00%,特异性为83.30%。结论:利用MALDI-TOF-MS结合WCX纳米磁珠技术可筛选出垂体腺瘤患者和健康对照成人血清蛋白质谱存在的差异蛋白质峰,可作为垂体腺瘤检测、随访的指标。

Objective： Application of matrix assisted laser desorption ionization time of flight mass spectrometry enhancement （ MALDI- TOF-MS） combined with WCX nanometer magnetic bead technique, screening of the serum biomarkers in pituitary adenoma, to establish a serum protein fingerprint classification decision tree. Methods： Analyse the serum samples of 40 cases of pituitary adenoma and 60 cases of healthy adult and find the different protein peaks, then to establish the diagnosis model and the classification decision tree of pituitary adenow_as. Results： A total of 42 differences in protein peaks were identified in the experimental and control group （ P 〈 0.01 ）. The diagnosis model of pituitary adenomas was established by three protein peaks （3382.0.4601.9.9191.2）. The model could screen the pituitary adenoma out of the normal population. The sensitivity was 90.00% and the specificity was 88.30%. By the double blind experimental validation, the model could diagnose the pituitary adenoma and the sensitivity was 88%, the specificity was 83.30%. Conclusion： Significantly different protein peaks can be screened out between pituitary adenoma cases and healthy controls using MALDI -TOF-MS combined with WCX technique, and these protein peaks may be used as a pituitary adenoma detection, follow-up indicator.