白花丹素对舌癌细胞Tca-8113放疗增敏的作用

Plumbagin Enhanced the Radiotherapy Sensitivity of Tongue Cancer Cell Line Tca-8113

目的:探讨白花丹素联合X-射线对舌癌细胞Tca-8113的放疗增敏作用及其机制。方法:体外培养舌癌细胞株Tca-8113,分空白对照组、单纯白花丹素组、单纯放疗组及白花丹素联合放疗组;应用MTT法检测白花丹素对舌癌Tca-8113细胞的毒性作用及最佳实验浓度;应用克隆形成确定白花丹素的放疗增敏效应;应用流式细胞仪检测各组细胞的凋亡率;采用Western Blot检测放射单独及联合白花丹素作用于Tca-8113细胞后,细胞核中NF-κB的蛋白表达水平的变化。结果:经MTT检测表明,白花丹素对舌癌Tca-8113生长抑制浓度IC10为1.42μmol/L(24h),即为最佳实验浓度;经克隆形成检测显示,白花丹素对舌癌Tca-8113细胞具有放疗增敏效应,增敏系数SER为1.28;流式细胞仪分析表明,白花丹素组与放疗组比较,其细胞早期凋亡率无明显差异,而两者联合作用后,舌癌细胞的早期凋亡率显著增高;Western Blot检测显示,白花丹素能够显著抑制放射对Tca-8113细胞核中NF-κB的激活作用。结论:白花丹素能够诱导舌癌Tca-8113凋亡并具有放疗增敏作用,抑制细胞中NF-κB的激活可能是其放射增敏的主要机制。

Objective： To study radiation sensitizing effect of Plumbagin on human tongue cancer cell line Tca-- 8113. Methods： The Tca--8113 ceils were cultured in vitro and randomized into control, radiation alone, Plumbagin alone and combination treatment group. MTT assay was employed to examine the growth status of the cells and de- termine the optimum dose for research. Clongenic assay was used to detect the survival of cells and confirm the radi ation sensitizing effect of plumbagin. The flow cytometry was applied to detect the apoptosis ratio of Tca--8113 cells in each group. Expression of nuclear factor-- kB was assessed by Western Blot. Results： The optimum dose of plumbagin on Tca--8113 ceils was 1.42μm/L for 24h. The clongenic assay suggested the enhancement of radiative sensitizion in virtue of plumbagin,SER was 1.28. Activation of NF-κB was repressed by Plumbagin. Conclusion.. Plumbagin can enhance the radiation sensitizing of human tongue cancer cell line Tca--8113. The repression of NF--κB might be the main mechanism of radiosensitivity enhancement.