人胎盘间充质干细胞成骨分化过程中免疫原性上调的研究

Study on upreguleted immunogenicity of human placenta-derived mesenchymal stem cells during osteogenesis

为探讨间充质干细胞在成骨分化过程中的免疫原性改变及其意义,利用酶消化法获得胎盘间充质干细胞(PMSC),经鉴定后诱导成骨,用流式细胞术检测细胞表面协同刺激分子的表达并与T细胞共培养,3 H-TdR掺入法检测T细胞的增殖。结果表明,PMSC成功向成骨细胞分化,未分化的PMSC不表达CD28、CD80、CD83、CD86等正性共刺激分子,但经成骨诱导分化后其表达上调,并刺激了T细胞增殖,而未诱导分化的PMSC则未能促进。因此,未分化的PMSC具有低免疫原性,但是在经成骨诱导分化后其免疫原性上调,这对间充质干细胞今后在临床的应用有一定指导意义。

To investigate the alteration of mesenchymal stem cells＇ immunogenicity during osteogenesis, human placenta de rived mesenchymal stem cells（PMSCs） were isolated, confirmed and induced into osteoblasts. The costimulatory molecules were detected by flowcytometer（FCM） after PMSCs were induced in osteogenic medium for 7 days. 3 H-TdR was used to detect the proliferation of T cells cocultured with PMSCs and differentiated PMSCs. Osteogenesis was confirmed by alizarin red stai- ning. The results showed that PMSCs differentiated into osteoblasts well. The induced cells upregulated the expressions of positive costimulatory molecules and they also promoted T cell proliferation. PMSCs are immuno-privileged in vitro. But their immunogenicity could be upregulated when osteogenic induced. This should be fully considered when MSCs are applied in clinic.