慢性睡眠障碍对免疫的抑制作用

Suppression of immune function in mice with chronic sleep disorder

分别采用连续4周喂养5%乙醇、连续14d水平台环境饲养法以及暗箱饲养模拟全夜状态等方法建立慢性睡眠剥夺模型,观察三种方法诱导睡眠障碍对小鼠免疫功能的抑制作用。通过脾脏称重、FACS法检测外周血CD3+、CD4+和CD8+T细胞数变化、ELISA法检测血清中IL-2、TNF-α以及B细胞产生抗牛血清白蛋白(BSA)特异性抗体含量、LDH法测定NK细胞杀伤功能等方法检测实验小鼠免疫功能变化。实验结果显示:与正常对照组相比,实验组免疫指标均有不同程度改变,包括CD3+、CD4+和CD8+T细胞数减少;IL-2分泌量下降和TNF-α分泌量升高;NK杀伤功能下降;脾重减轻;抗BSA特异性抗体分泌量下降。实验证明,长期5%乙醇摄入、水平台以及暗环境干扰引起的慢性睡眠障碍对机体的免疫功能具有明显抑制作用。

To investigate the effects of chronic sleep disorder on immune function, sleep restriction was imposed on female mice by housing them respectively in a completely dark environment and in water platform for 14d or by feeding them with 5 0/oo alco- hol for 4 weeks. Count of T subset cells was detected by FACS. Serum levels of IL 2, TNF-a and antibodies to BSA were measured by ELISA. The NK activity was determined by LDH release method and the spleens were weighted. The results showed that compared with the normal control group, immunologic indexes in experimental group were changed significantly in varying degrees, include the number of CDa＋ , CD4＋ , CD8＋ T cell decreased, serum level of IL-2 and NK activity declined, serum level of TNF-α elevated, spleen weight of the mice reduced and serum concentration of specific antibody to BSA de- creased. The data approved that long term sleep restriction induced by alteration of circadian rhythmicity or alcohol could result in a significant impairment in immune function.