ADAM33基因S2位点多态性与新疆维吾尔族人群哮喘的相关性研究

Association between Polymorphism of S2 Locus Allele in ADAM33 Gene and Bronchial Asthma in Xinjiang Uygur Population

目的探讨解整合素-金属蛋白酶33基因S2位点多态性与新疆维吾尔族人群支气管哮喘的相关性。方法纳入131例新疆维吾尔族支气管哮喘患者(病例组)与90例新疆维吾尔族健康体检者(对照组),应用聚合酶链和限制片段长度多态性(PCR-RFLP)方法分析ADAM33基因S2位点单核苷酸多态性。结果 ADAM33基因S2位点3种基因型比较差异有统计学意义(x2=6.065,P<0.05);2组等位基因频率比较差异有统计学意义(x2=5.255,P<0.025);S2位点G等位基因有增加患哮喘的风险(OR=1.616,P<0.05);S2位点的CG基因型有增加患哮喘的风险(OR=1.351,P<0.05),哮喘组ADAM33基因S2位点3种基因型间FEV1%pred和FVC%pred比较差异有统计学意义(F=6.248,P<0.01;F=7.067,P<0.01)。结论 ADAM33基因S2位点与新疆维吾尔族支气管哮喘具有明显相关性,并且增加维吾尔族人群患哮喘的风险。

Objective To investigate the association between polymorphism of $2 l（~eus allele in ADAM33 gene and bronchial asthma in Xinjiang Uygur population. Methods PCR-RFLP was used to determine polymorphism of S2 locus allele in ADAM33 gene in 131 Uygur patients with bronchial asthma （asthma group） and 90 Uygur healthy individuals （control group）. Results The comparison of three genotypes and allele frequency of the S2 in the ADAM33 gene had statistical significance in the asthma group and the control group （ X2 = 6. 065,P 〈 0. 05 ;Z2 = 5. 255,P 〈 0. 025 ）. The G allele of $2 site increased the risk of asthma（ OR = 1. 616,P 〈0. 05 ）. The CG genotype also increased the risk of asthma （ OR = 1. 351 , P 〈0. 05）. The FEV1% pred and FVC% pred had significant difference between three genotypes of the S2 site in the ADAM33 gene in the asthma group （ F = 6. 248, P 〈 0. 01 ; F = 7. 067, P 〈 0. 01 ）. Conclusion The polymorphism of the S2 site in the ADAM33 gene has significant correlation with asthma in Xinjiang Uygur population,and can increase risk of asthma in the Uygur population.