K粉相关性膀胱炎患者膀胱组织中组胺受体的表达及临床意义

The Expression and Clinical Significance of Histamine Receptors in Urinary Bladder in Patients with Ketamine-induced Cystitis

目的研究4种组胺受体H1R、H2R、H3R和H4R在K粉相关性膀胱炎患者膀胱组织中的表达及肥大细胞浸润的临床意义。方法采用免疫组化S-P法检测10例K粉相关性膀胱炎患者膀胱组织(研究组)及10例膀胱全切患者远离膀胱肿物的正常膀胱组织(对照组)中组胺受体H1R、H2R、H3R和H4R的表达,检测2组4种不同组胺受体平均光密度(OD值),甲苯胺蓝特殊染色标记2组标本中肥大细胞并计数。结果与对照组比较,研究组H1R、H2R和H4R的表达明显上调,肥大细胞在膀胱组织间浸润增多(均P<0.05),2组膀胱组织中H3R的表达差异无统计学意义。结论肥大细胞以及H1R、H2R和H4R与K粉相关性膀胱炎关系密切,并可能成为诊断该病的新指标和治疗的新靶点。

Objective To investigate the expression and clinical significance of four histamine receptors： HtR, H2R, H3R and H4R in urinary bladder of patients with ketamine-induced cystitis. Methods Immunohistochemical S-P method was used to detect the expression levels of histamine receptors： H^R, H2R, H3R and H4R in bladder tissues of 10 patients with ketamine-induced cystitis （experimental group） and distal tissue away from bladder tumors of 10 patients with eystectomy （control group）. The average optical density （OD） values of four kinds of different histamine receptors were separately calcu- lated by Imagepro-plus 6.0 in two groups. At the same time, mast cells were marked by toluidine blue special dyeing and were counted. Results Comparing with control group, the expression levels of H^R, H2R, and H4R were significantly in- creased in experimental group （P 〈 0.05）. Mast cells diffused interstitial bladder infiltrates （P 〈 0.05）. There was no signifi- cant difference in the expression of H3R in two groups. Conclusion Mast cells, H R, H2R, and H4R are closely related to the ketamine-induced cystitis, which may be new diagnostic indicators and new treatment targets of ketamine-induced cysti- tis.