摘要
目的提取西安地区出土3000年前人牙齿中的DNA并进行性别鉴定，为后续的考古研究提供帮助。方法选取西安地区出土的3000年前人牙齿35颗，应用逆向根管技术提取牙齿内部组织的DNA。设计釉原蛋白基因特异性引物，应用PCR技术进行样本的性别鉴定。结果成功提取到30份3000年前的人基因组DNA，并对相应样本进行了性别鉴定，其中男性8例，女性22例。结论逆向根管技术可用于古人类牙齿DNA的提取，基因学方法可补充体质人类学方法的不足，完善古代样本性别信息。
Objective To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi＇an and determine the genders for the individuals. Methods Thirty-five ancient human teeth were studied. A ＇Reverse-root-canal＇ technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification. Results Genomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females. Conclusion The ＂Reverse-root-canal＂ technique can be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for sex-determination of ancient samples.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2013年第5期619-621,共3页
Chinese Journal of Medical Genetics
基金
陕西省社发攻关项目（2011K15-06-04）
