洋葱组培快繁技术研究

Study on the Tissue Culture and Rapid Propagation Technology of Allium cepa L.

以"玉黄"洋葱鳞茎盘为外植体,以MS为基本培养基,采用L16(24)正交实验设计,研究了6-BA、NAA及IBA不同浓度及组合对洋葱组培快繁的影响。结果表明:6-BA及NAA在诱导洋葱愈伤组织、不定芽时交互作用显著;洋葱最适愈伤组织诱导培养基为MS+NAA 0.10mg/L+6-BA 2.00mg/L;最适不定芽诱导培养基为MS+NAA 0.05mg/L+6-BA 1.50mg/L+多效唑(MET)0.25mg/L;最适不定根诱导培养基为1/2MS+IBA 1.0mg/L。

Taking the basal plates of Allium cepa ＇Huangyu＇ as explants,the effect of different types and concentrations of growth hormone on callus induction,adventitious bud germination and plantlets rooting were studied through L16 （24） orthogonal experiments,with MS as the basic medium. The results showed the best culture medium of callus induction of Allium cepa basal plate was MS＋ NAA 0. 10 mg/L＋ 6-BA 2. 00 rag/L; the best culture medium of adventitious bud germination was MS＋NAA 0. 05 mg/L＋ 6-BA 1.50 rag/L＋ MET 0. 25 mg/L; the best culture medium of ptantlets rooting was 1/2MS＋IBA 1. 0 mg/L.