橡皮树组织培养及试管苗的温室移栽

Tissue Culture of Ficus elastic Roxb and the Transplanting of Plantlets in Greenhouse

以橡皮树"黑金刚"茎尖为试材,研究了不同激素水平对橡皮树愈伤组织诱导、丛生芽诱导、增殖和生根的影响,并对橡皮树试管苗的温室移栽技术进行了总结。结果表明:橡皮树丛生芽诱导的最适培养基为MS+6-BA 1.0mg/L+NAA 0.01mg/L;丛生芽增殖的最适培养基为MS+6-BA 3.0mg/L+NAA 0.01mg/L,增殖倍数可达3.6倍;生根培养的最适培养基为1/2MS+IBA 1.0mg/L。在温室移栽时要注意基质的消毒和环境的控制,移栽成活率可达到90%以上,显著提高经济效益。

Taking Ficus elastica Roxb ＇Heijingang~ as material,the effect of different concentrations of hormone on callus induction,multiple shoot induction and proliferation, rooting of it were studied, and the transplanting technology of plantlets in greenhouse was summarized. The results showed that the optimum medium for multiple shoot induction was MS^6-BA 1. 0mg/L＋NAA 0. 01 mg/L. For multiple shoot proliferation was MS＋6-BA 3. 0 mg/L＋NAA 0. 01 rag/L, with proliferation multiple 3. 6, and rooting medium was 1/2MS＋ IBA 1.0 mg/L. The transplanting survival rate can reach more than 90 % if environmental was controlled and the substrate was sterilized in greenhouse. It could significantly improve the economic benefits.