摘要
以香菇子实体为原料,采用Plackett-Burman设计对影响香菇多酚提取效率的8个因素进行筛选,并通过Box-Behnken设计及响应面分析法确定了影响多酚类提取因素的最佳条件。结果表明:影响香菇多酚提取的3个主要因素为:乙醇浓度、液料比和提取温度;最佳提取条件为:香菇过160目筛,以81%的乙醇作溶剂,液料比为34∶1mL/g,先超声处理15min,在85℃的水浴中提取45min,提取3次。根据最优试验条件对模型进行验证,得到香菇子实体的多酚提取量为186.50mg/100gFW,实际值与预测值的误差为3.24%。
Taking the fruit of Lentinus edodes as material, Plackett-Burman design was used to evaluate the influence of related eight factors of total phenolic from Lentinus edodes. And the Box-Behnken design and response surface analysis were used to determine the optimal levels of the main factors. The results showed that three factors played the important roles in the medium, including ethanol concentration, temperature and solvent solid ratio. The optimal conditions were particle size less than 160 pore, 15 min for ultrasonic time, 34 .： 1 mL/g for solvent solid ratio,81 ethanol for solution, extracted at 85℃ for 45 min,extracting for 3 times. Under the optimal conditions, the yield of total phenolic reached 186. 50 mg/100gFW,only 3. 24~ differences from the forecast by RSM.
出处
《北方园艺》
CAS
北大核心
2013年第18期125-128,共4页
Northern Horticulture
基金
嘉兴市科技计划资助项目(2012AY1058)
关键词
香菇
多酚
响应面分析法
优化
Lentinula edodes
total phenolic
response surface methodology
optimization
