竹纤维制备的预处理方式比较及纤维素酶筛选

Effects comparison of different pretreatment methods and screening of cellulase on bamboo fibre processing

为了实现以生物方法制备竹纤维,本文以竹粉为研究对象,分析了几种纤维素酶吸附特性以及不同预处理方法对竹粉作用效果影响。实验表明,纤维素酶与竹粉的酶解作用发生在酶吸附竹纤维之后,纤维素酶的吸附在反应开始30min内较为明显,并逐渐达到饱和;通过对竹粉有机抽提、有机抽提后高温处理和有机抽提后超声波处理三种不同预处理方法发现,在相同酶处理工艺条件下,以紫外/可见分光光度计测定还原糖产量,发现采用有机溶剂加高温处理方式效果最好;研究了经不同时间的高温处理,以15min处理时间最为合适。

In order to achieve bamboo fibre through biochemical approach, the characters of cellulase adsorption on bamboo powders and the influence of different pretreatments on bamboo processing were analyzed. The obtained results indicated that cellu lase can degrade part of the cellulose in bamboo after its adsorption. It can be seen that the cellulase started to be adsorbed on bam boo substrates within the first 30minutes and then saturated gradually with the prolonging of the tim In addition, three pretreat ments, i. high temperature pretreatment on organic solvent treated bamboo, ultrasound pretreatrnent on organic solvent treated bamboo, organic solvent pretreatment on original bamboo, were applied to investigate their influence on cellulase processing respec-tively. The results from UV spectrophotometer demonstrated that the highest reducing sugar was achieved when the organic solvent and high temperature pretreatment was applied. Furthermore, by comparing different times of high temperature pretreatment on bamboo, it shown that 15min led to the best performance.