HPLC-ELSD测定不同产地预知子中四种皂苷的含量

Quantitative Determination of Four Components of Akebiae Fructus from Different Habitats

目的：采用HPLC-ELSD测定预知子中常春藤皂苷元3-O-β-D-吡喃葡萄糖基-（1→3）-α-L-吡喃鼠李糖基-（1→2）-α-L-吡喃阿拉伯糖苷（皂苷X,saponins X）,常春藤皂苷元3-O-β-D-吡喃木糖基-（1→2）-α-L-吡喃阿拉伯糖苷（皂苷B,saponins B）,常春藤皂苷元3-O-α-L-吡喃鼠李糖基-（1→2）-α-L-吡喃阿拉伯糖苷（皂苷P D,saponins P D）,常春藤皂苷元3-O-α-L-吡喃阿拉伯糖苷（皂苷A,saponins A）的含量。方法：采用安捷伦1100手动高效液相色谱仪,色谱柱为Cosmosil-C18ODS色谱柱（4.6mm×250 mm,5μm）,ELSD检测器,流动相乙腈-0.2%甲酸水（45∶55）,体积流量1.0 mL·min-1;蒸发光散射检测器检测参数：漂移管温度98℃,氮气流量2.8 L·min-1。结果：皂苷X,皂苷B,皂苷P D,皂苷A进样量在2~10μg线性关系良好（R2分别为0.999 0,0.999 0,0.999 1,0.999 1）。平均回收率（n=6）分别为96.8%,101.9%,99.3%,100.5%;RSD分别为0.9%,1.2%,0.7%,1.3%。结论：该方法简单,分离效果好,结果准确,可用于预知子药材中皂苷X,皂苷B,皂苷P D,皂苷A的含量测定。

Objective： To establish a method for the determination of the content of 3-O-β-Dglucopyranosyl（1→3）-α-L-rhamnopyranosyl-（1→2）-α-L-arabinopyranosyl hederagenin（saponins X）,3-Oβ-D-xylopyranosyl-（1→2）-α-L-arabinopyranosyl hederagenin（saponins B）,3-O-α-L-rhamnopyranosyl-（1→ 2）-α-L-arabinopyranosyl hederagenin（saponins P D）,3-O-α-L-arabinopyranoside hederagenin（saponins A） in Akebiae Fructus using high-performance liquid chromatography with evaporative light scattering detector（HPLCELSD）.Method： The separation of A.Fructus was performed on an Agilent 1100 manual high performance liquid chromatograph,Cosmosil-C 18 ODSchromatography column（4.6 mm × 250 mm,5 μm）.The mobile phase was acetonitrile-water and the flow rate was 1.0 mL·min-1.The detector was ELSD.The temperature of drift tube was 98 ℃.And the nebulizer nitrogen flow rate was 2.8 L·min-1.Result： The linear range of saponin X,saponin B,saponins P D,saponin A was 2-10 μg（R2was 0.999 0,0.999 0,0.999 1,0.999 1,respectively）,and the average recovery（n = 6） was 96.8%,101.9%,99.3%,100.5%,respectively;and RSD was 0.9%,1.2%,0.7%,1.3%,respectively.Conclusion： The method is simple,reliable and accurate.It can be used to determine saponins X,saponins B,saponins P D,saponins A content and control the quality in Akebiae Fructus.