不同来源甘草药材成分含量及其保肝降酶作用比较

Comparative Study on Major Ingredient Contents and Effect of Depressing Transaminase of Licorice from Different Sources

目的:研究不同来源甘草药材的成分含量及其保肝降酶作用。方法:采用HPLC同时测定甘草成分含量;用醋氨酚复制肝损伤模型,灌胃不同来源甘草的相同提取物,测定血清丙氨酸转氨酶(ALT)和谷氨酸转氨酶(AST)活力。结果:不同来源甘草组的ALT,AST活力与模型组比较具有显著性差异(P<0.01);S1与S8甘草组之间的ALT,AST活力没有显著性差异,保护率分别为97.9%,95.8%,98.1%,94%,但前者甘草酸、甘草苷、异甘草素的含量却明显高于后者,分别为2.219%,2.86%,0.023%和0.767%,0.557%,0.013%;S4和S6甘草的保肝能力最差,保护率分别为67.4%,65.6%和70.1%,64.5%,而甘草酸、甘草苷含量却明显高于其他产地三年栽培甘草。结论:不同来源甘草对肝损伤小鼠的肝功能都有不同程度的保护作用,并存在一定的质量差异,但与甘草酸、甘草苷的含量没有呈现正相关性,提示甘草保肝降酶作用可能是甘草药材中多种成分相互作用的结果。

Objective： To study the difference of Licorice from different sources in major ingredient contents and protective effect on liver.Method： HPLC was applied to determine the contents of Glycyrrhizin,liquiritin and isoliquiritigenin from Licorice,and mice were chosen to duplicate the acetaminophen-induced toxic hepatitis model.Result： Licorice was able to significantly decrease serum transaminase levels of chemical liver injury＇s mice induced by acetaminophen.Difference of serum transaminase activity between S1 and S8 groups was not significant,but the contents of Glycyrrhizin,liquiritin and isoliquiritigenin from S1 were more higher than them from S8,which were 2.219%,2.86%,0.023% and 0.767%,0.557%,0.013% respectively.The protective effect on liver of S4 and S6 were relatively weak,but their contents of constituents were still higher.Conclusion： The quality of licorice from different sources is different,and the protective effect on liver has no positive relationship with contents of glycyrrhizin,liquiritin and isoliquiritigenin.Those show that it could be related to interreaction of various chemical constituents of Licorice.