清肺消痤颗粒的薄层鉴别研究

TLC Method of Qingfeixiaocuo Granules

目的:建立清肺消痤颗粒的薄层鉴别方法,为质量标准的制定提供依据。方法:采用薄层色谱法对清肺消痤颗粒中黄芩、丹参、赤芍等所有饮片进行定性鉴别。结果:分别以含水量75%的微乳-甲酸(9.5∶0.5),三氯甲烷-乙酸乙酯-甲醇-甲酸(40∶5∶10∶0.2),苯-乙酸乙酯(19∶1),石油醚-甲酸甲酯-甲酸(15∶5∶1)氯仿-甲醇(20∶1),氯仿-乙醚(1∶1),苯-丙酮(1∶1)为黄芩、赤芍、丹参、虎杖、连翘、桔梗、浙贝母的展开剂,以甲苯-乙酸乙酯-甲酸(20∶4∶0.5)为山楂和桃仁的展开剂;在聚酰胺薄膜或者硅胶板上展开,所得的薄层色谱斑点清晰,特征明显,阴性对照未见干扰。结论:所建立的方法专属性强,重复性好,可用于清肺消痤颗粒的定性鉴别。

Objective： To establish identification method of Qingfeixiaocuo Granules by TLC and provide experimental basis for the quality control.Method： TLC was used to distinguish all Chinese herbal medicine in the prescription which contains Scutellaria baicalensis,Salvia miltiorrhiza and Radix Paeoniae Rubrae and so on.Result： 75% content of water microemulsion-formic acid（9.5 ∶ 0.5）,chloroform-ethyl acetate-methanol-formic acid（50∶5 ∶ 10 ∶ 0.2）,benzene-ethyl acetate（19 ∶ 1）,petroleum ether-methyl formate-formic acid（15 ∶ 5 ∶ 1）,chloroform-methanol（20∶1）,chloroform-diethyl ether（1 ∶ 1）,benzene-acetone（1 ∶ 1） were used as developing agent for Scutellaria,Radix Paeoniae Rubra,Radix Salviae Mitiorrhizae expansion agent,Polygonum cuspidatum,Forsythia,Radix Platycodi,Bulbus Fritillariae Thunbergii respectively,and toluene-ethyl acetate-formic acid（20∶ 4∶0.5） as the agent for hawthorn and wainut,which launched on the polyamide film or silica gel plate.The spots of TLC of all varieties were distinctive and there was no interfere with the negative reference.Conclusion： The methods are specific and reliable that can be used as qualitative identification for Qingfeixiaocuo Granules.