HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量

Determination of Aconitine,Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC

目的:建立复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量测定方法。方法:采用HPLC,流动相甲醇-0.5%的三乙胺(70∶30),Aglient TC-C18色谱柱(4.6 mm×250 mm,5μm),检测波长235 nm,流速1.0 mL·min-1,柱温室温。结果:乌头碱的进样量在0.080 8~0.808 0μg具有良好的线性关系(r=0.999 6),平均回收率98.40%,RSD 2.84%;新乌头碱的进样量在0.866~4.330μg具有良好的线性关系(r=0.999 7),平均回收率98.04%,RSD 2.05%;次乌头碱的进样量在0.089~0.890μg具有良好的线性关系(r=0.999 8),平均回收率98.71%,RSD 2.34%。结论:方法操作简便、灵敏度高、结果准确,可作为复脉灵胶囊的含量测定方法。

Objective： To .establish a method for the determination of aconitine,, mesaconitine and hypaeonitine in Fumailing Capsules. Method： The aconitine, mesaeonitine and hypaeonitine in the capsules was determined by HPLC on the Aglient TC-Clscolumn （4.6 mm x 250 mm, 5 ixm） at room temperature. The mobile phase was consisted of methanol-0.05% triethylamine （70 ： 30）. The flow rate was 1.0 mL ~ min-1 and the detection wavelength was set at 235 nm. Result： The content of aeonitine had a good linearity in the range of 0. 080 8-0. 808 0 p^g （ r = 0. 999 6） , the average recovery was 98.40% , RSD was 2.84%. The content of mesaconitine had a good linearity in the range of 0. 866-4. 330 ixg （r = 0. 999 7） , the average recovery was 98.04% and RSD was 2.05%. The content of hypaeonitine had a good linearity in the range of 0. 089-0. 890 p.g （r =0. 999 8）, the average recovery was 98.71% , and RSD was 2.34% ; Conclusion： This method was simple, reproducible and accurate, and it can be used for determination of Fumailing Capsules.