HPLC测定降粘胶囊中绿原酸的含量

HPLC Determination of Chlorogenic Acid in Jiangnian Capsules

目的：建立测定降粘胶囊中绿原酸含量的高效液相色谱法。方法：采用Phenomenex C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-0.2%磷酸（20∶80）,流速1.0 mL·min-1,检测波长327 nm,进样量10μL,柱温30℃。结果：绿原酸质量浓度在4.344~130.3 mg·L-1（r=0.999 8）与峰面积呈良好线性关系,平均加样回收率为99.93%（RSD 0.12%）。结论：方法测定降粘胶囊中绿原酸的含量,简便、准确,结果稳定,可为降粘胶囊的质量评价提供依据。

Objective： To determine the content of chlorogenic acid in Jiangnian Capsules by HPLC.Method： Phenomenex C 18 column（4.6 mm × 250 mm,5 μm） was used.The mobile phase was methanol-0.2% phosphoric acid（20∶ 80） and the detecting wavelength was set at 327 nm;the flow rate was 1.0 mL·min-1,and the injection volume was 10 μL;the column temperature was kept at 30 ℃.Result： The linear range of calibration curve was 4.344-130.3 mg·L-1for chlorogenic acid（r = 0.999 8）.The average recovery rate was 99.93%（RSD 0.12%）.Conclusion： The method is highly sensitive,quick and accurate with good repeatability,which can be used for the quality control of Jiangnian Capsules.